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| 产品名称： | pSVBV-E3 |
|---|---|
| 商品货号： | TS204472 |
| Designations： | pSVBV-E3 |
| Species： | strawberry vein banding caulimovirus |
| Depositors： | TJ Morris |
| Applications： | Non-infectious full-length clone of strawberry vein banding virus useful in diagnosing the infection. |
| Vector： | Construct size (kb): 10.5 |
| Insert： | DNA: genomic Insert lengths(kb): 7.800000190734863 |
| Insert Size (kb)： | 7.800 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | (BamHI-290-) HindIII-1050- XbaI-875- HindIII-100- AvaI-125- XhoI-570- XbaI-190- HindIII-100- XbaI-100- HindIII-190- EcoRI. The PstI, KpnI and BamHI sites are unique within the insert. Insert contains the following sites separated by (bp)(approx): EcoRI-475- XbaI-140- HindIII-1050- HindIII-170- EcoRV-50- NcoI-70- AvaI-100- XhoI-190- PstI-570- HindIII-190- NcoI-190- HindIII-620- EcoRV-100- AvaI-50- KpnI-30- EcoRV-290- BamHI-290-. Restriction digests of the clone give the following sizes (kb): PstI--9.4, 2.4; BamHI--6.8, 5.1; EcoRI--8.6, 3.0. Distribution and titer of virion DNA varied greatly between leaflets from the same infected plant. Non-infectious full-length clone of strawberry vein banding virus useful in diagnosing the infection. Isolated from Fragaria vesca. Purified virion DNA digested with EcoRI and cloned into EcoRI site of pUC8. Digested recombinants that co-migrated with full-length linear virion DNA were further screened by Southern blot. |
| Classification： | Caulimovirus |
| References： | Stenger DC, et al. Isolation, molecular cloning, and detection of strawberry vein banding virus DNA. Phytopathology 78: 154-159, 1988. |