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微信小章| 产品名称： | p22-phox-cDNA |
|---|---|
| 商品货号： | TS204507 |
| Designations： | p22-phox-cDNA |
| GenBank Number： | J03774 |
| Species： | Homo sapiens, human |
| Depositors： | SH Orkin |
| Vector： | Construct size (kb): 3.400000095367432 |
| Insert： | DNA: cDNA Insert lengths(kb): 0.6800000071525574 Tissue: HL-60 cell line (promyelocytic leukemia) Gene product: cytochrome b-245, alpha polypeptide CYBA |
| Insert Size (kb)： | 0.680 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--2.7, 0.7; HindIII--3.4; EcoRI/HindIII--2.7, 0.7; BamHI--3.4; PstI--3.4. Detects human genomic fragments of 13, 11 and 1.2 kb, well separated from two mouse fragments of intermediate size. The insert begins 3 to the NcoI site at nt 35 of the published sequence and extends through the poly(A) tail. |
| References： | Bu-Ghanim HN, et al. The alpha subunit of cytochrome b-245 mapped to chromosome 16. Genomics 8: 568-570, 1990. PubMed: 2286377 Dinauer MC, et al. Human neutrophil cytochrome b light chain (p22-phox). Gene structure, chromosomal location, and mutations in cytochrome-negative autosomal recessive chronic granulomatous disease. J. Clin. Invest. 86: 1729-1737, 1990. PubMed: 2243141 Parkos CA, et al. Primary structure and unique expression of the 22-kilodalton light chain of human neutrophil cytochrome b. Proc. Natl. Acad. Sci. USA 85: 3319-3323, 1988. PubMed: 3368442 Stuart H Orkin, personal communication |