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微信小章| 产品名称： | pHcGAP |
|---|---|
| 商品货号： | TS204629 |
| Designations： | pHcGAP |
| GenBank Number： | M17851 |
| Species： | Homo sapiens, human |
| Applications： | standard for analysis of gene expression glyceraldehyde-3-phosphate dehydrogenase glyceraldehyde 3-phosphate dehydrogenase, phosphoglyceraldehyde dehydrogenase, triosephosphate dehydrogenase |
| Vector： | Construct size (kb): 5.599999904632568 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.200000047683716 Tissue: fetal liver Gene product: glyceraldehyde-3-phosphate dehydrogenase(glyceraldehyde 3-phosphate dehydrogenase, phosphoglyceraldehyde dehydrogenase, triosephosphate dehydrogenase) GAPD Target Gene: glyceraldehyde-3-phosphate dehydrogenase |
| Insert Size (kb)： | 1.200 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | The PstI site at the 3 end of the insert was destroyed during cloning. The retained PstI site is 750 bp from the EcoRI site. The insert contains a HindIII and a XbaI site. Restriction digests of the clone give the following sizes (kb): PstI--5.55; HindIII--4.5, 1.06; RsaI--2.1, 1.4, 0.86, 0.65, 0.56; XbaI/PstI--4.7, 0.8; BglI--2.9, 2.2, 0.4, 0.2; BglI/PstI--2.2, 1.8, 1.0, 0.4, 0.2. Includes 31 bp of 5 and 216 bp of 3 untranslated sequence, and a complete 1008 bp coding sequence. The polyadenylation signal AATAA is 15 bp upstream from poly(A) site. The XbaI site is about 780 bp from the 5 PstI site. A PstI/XbaI double digest should give a probe containing the 5 non-coding sequences and sequences encoding about the first 250 amino acids. Recorded as not polymorphic by Collaborative Research, meaning either not polymorphic in 0.8% agarose gels, or difficult to work with. Thirty hybridizing bands are detected in genomic blots at 37C, 50% formamide, 5X SSC; 100 bands are detected when screening a genomic library under the same conditions. |
| References： | Tso JY, et al. Isolation and characterization of rat and human glyceraldehyde-3-phosphate dehydrogenase cDNAs: Genomic complexity and molecular evolution of the gene. Nucleic Acids Res. 13: 2485-2502, 1985. PubMed: 2987855 Norgard MV, et al. Activation of human monocytic cells by Treponema pallidum and Borrelia burgdorferi lipoproteins and synthetic lipopeptides proceeds via a pathway distinct from that of lipopolysaccharide but involves the transcriptional activator NF-kappaB. Infect. Immun. 64: 3845-3852, 1996. PubMed: 8751937 Filler SG, et al. Candida albicans stimulates cytokine production and leukocyte adhesion molecule expression by endothelial cells. Infect. Immun. 64: 2609-2617, 1996. PubMed: 8698486 Barbara Weiffenbach, personal communication |