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| 产品名称： | Dr-1 |
|---|---|
| 商品货号： | TS204676 |
| Designations： | Dr-1 |
| GenBank Number： | M97388 |
| Species： | Homo sapiens, human |
| Vector： | Construct size (kb): 4.349999904632568 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBluescript SK- Intact vector size: 2.960 Type of vector: phagemid Vector end: EcoRI Vector end: EcoRI Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI (Eco0109I DraII ApaI) KpnI Construction: pUC19 Host range: Escherichia coli Features (with orientation and position when available): replicon: f1, -> insert detection: lacZ, primer site: M13-20, -> promoter: T7, -> MCS: KpnI...SacI, -> promoter: T3, promoter: lac, primer site: M13 reverse, replicon: pMB1 marker(s): ampR, Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: DR1* Genomic copy number: unique Gene name: transcription repressor (interacting with the TATA-binding protein) Contains complete coding sequence?: Y Tissue: HeLa cell line Type of DNA: cDNA Insert end: Modification: EcoRI linkers Insert end: Modification: EcoRI linkers Insert size (kb): 1.4 Cross references: DNA Seq. Acc.: M97388 Insert lengths(kb): 1.399999976158142 Tissue: HeLa cell line Gene product: transcription repressor (interacting with the TATA-binding protein) DR1* |
| Insert Size (kb)： | 1.400 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 0.9, 0.74; KpnI--3.3, 1.4; SstI--4.6; PvuII--2.6, 0.9, 0.72, 0.58; XbaI--3.2, 0.94, 0.58; BamHI/HindIII--2.9, 1.70. The insert contains the following restriction sites (approximate kb from the 5 end): EcoRI--0.68; KpnI--0.14, 0.15; SmaI--0.44; PvuII--0.40, 1.09; XbaI--0.32, 1.2. Detects transcripts of 3.4 and 1.5 kb. Transcription from the T7 promoter gives the sense strand. |
| References： | Inostroza JA, et al. Drl, a TATA-binding protein-associated phosphoprotein and inhibitor of class II gene transcription. Cell 70: 477-489, 1992. PubMed: 1339312 Juan A Inostroza, personal communication |