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| 产品名称： | Perkinsus marinus (Mackin et al.) Levine |
|---|---|
| 商品货号： | TS204678 |
| Strain Designations： | C13-9 MA-2-9 |
| Application： | This strain is useful for studies of population genetics, systematics, and virulence. |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | clone derived from strain C13 (ATCC 50783), which came from eastern oyster, Crassotrea virginica, Cotuit, MA, 1998 |
| Product Format： | frozen |
| Type Strain： | no |
| Comments： | parasite of eastern oyster, Crassotrea virginica, and possibly several other marine bivalves This strain is useful for studies of population genetics, systematics, and virulence. |
| Medium： | ATCC® Medium 1886: Perkinsus broth medium |
| Growth Conditions： | Temperature: 25.0°C Duration: axenic Protocol: ATCCNO: 50439 SPEC: When the frozen ampule arrives, place it directly into a 35C-water bath and transfer its thawed contents to 14 ml of fresh medium in a T-75 tissue culture flask. Maintain by removing 13 ml of cell suspension weekly and replacing with an equal volume of fresh medium. Alternately, aseptically transfer 1.0 ml of a growing culture to 13 ml of fresh medium in a T-75 tissue culture flask. Please note: This strain has wide tolerances to most environmental variables, i.e., temperature range: 15-35C, salinity range: 10-60 parts per thousand; pH range: 6.0-8.5. The distribution of the species worldwide is not known. In order to avert the introduction of this pathogen into non-endemic areas, all culture wastes must be treated as biohazardous and autoclaved prior to disposal. There are no known mechanisms for eradication of this pathogen from the environment. |
| Subcultivation： | Protocol: ATCCNO: 50439 SPEC: When the frozen ampule arrives, place it directly into a 35C-water bath and transfer its thawed contents to 14 ml of fresh medium in a T-75 tissue culture flask. Maintain by removing 13 ml of cell suspension weekly and replacing with an equal volume of fresh medium. Alternately, aseptically transfer 1.0 ml of a growing culture to 13 ml of fresh medium in a T-75 tissue culture flask. Please note: This strain has wide tolerances to most environmental variables, i.e., temperature range: 15-35C, salinity range: 10-60 parts per thousand; pH range: 6.0-8.5. The distribution of the species worldwide is not known. In order to avert the introduction of this pathogen into non-endemic areas, all culture wastes must be treated as biohazardous and autoclaved prior to disposal. There are no known mechanisms for eradication of this pathogen from the environment. |
| Name of Depositor： | D Bushek |
| Chain of Custody： | ATCC < |
| Year of Origin： | 1998 |