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| 产品名称： | pAL-14 |
|---|---|
| 商品货号： | TS204717 |
| Designations： | pAL-14 |
| Species： | Rattus norvegicus, rat |
| Depositors： | M Barbacid |
| Applications： | Two are copies of a HpaII/SacI fragment derived from 5 flanking sequences and used as a probe to detect the diagnostic MnlI RFLP. The HpaII/HpaII fragment is of unknown origin. This clone has been used to detect the MnlI RFLP indicative of a G to A mutation in codon 12 of the rat Ha-ras-1 locus induced by N-nitroso-N-methylurea. |
| Vector： | Construct size (kb): 3.099999904632568 |
| Insert： | DNA: genomic Insert lengths(kb): 0.3600000143051148 Tissue: mammary tumor Gene product: Harvey rat sarcoma viral (v-Ha-ras) oncogene homolog(Harvey rat sarcoma virus oncogene) HRAS |
| Insert Size (kb)： | 0.360 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Contains three 120 bp fragments. Two are copies of a HpaII/SacI fragment derived from 5 flanking sequences and used as a probe to detect the diagnostic MnlI RFLP. The HpaII/HpaII fragment is of unknown origin. This clone has been used to detect the MnlI RFLP indicative of a G to A mutation in codon 12 of the rat Ha-ras-1 locus induced by N-nitroso-N-methylurea. The normal allele is 206 bp and the mutant is 280 bp. |
| References： | Zarbl H, et al. Direct mutagenesis of Ha-ras-1 oncogenes by N-nitroso-N-methylurea during initiation of mammary carcinogenesis in rats. Nature 315: 382-385, 1985. PubMed: 3923365 |