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| 产品名称： | lambdaSK17 clone 2731A |
|---|---|
| 商品货号： | TS204722 |
| Designations： | lambdaSK17 clone 2731A |
| Depositors： | ER Zabarovsky, G Winberg, G Klein |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 51.0000000000000000 Vector: lambdaSK17 (phage, lambda - replacement) Promoters: Promoter lac Marker(s):red+ gam+ Construct size (kb): 51.0 Features: insert detection: Spi+ marker(s): red+ gam+ promoter: lac replicon: lambda, pMB1, M13 |
| Applications： | expression vector vector for constructing cDNA libraries vector permitting positive selection for inserts |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--25.0, 13.5 (doublet); EcoRI--25.0, 13.5 (doublet); SalI--21.0, 8.8, 8.2, 5.0, 4.4, 3.6. Expression vector permitting positive selection (Spi) of recombinants and control of expression using ochre (supB, supC and/or supG, supV) and amber (supE, supF) suppressors. Useful for conversion to phagemid. Permits expression in three reading frames, construction using partial filling-in, and oriented cloning. The restriction sites to be used for conversion to phagemid are: SalI, ClaI. Cloning capacity: 0.2 - 15.4 kb. The sizes of the major components are (kb): arms--20.3, 12.7; stuffer fragment--13.7; phagemid segments--3.9 (longer arm), 0.7. The order of the major features in this plasmid is: arm - ClaI - BspMII - SalI - pMB1 ori - ampR - M13 ori - SacI/MCS/ SalI --> - stuffer - SalI/MCS/XbaI/lacZ - ClaI - SalI - arm. |
| References： | Zabarovsky ER, et al. Lambda SK diphasmids: phage lambda vectors for genomic, jumping, linking and cDNA libraries. Gene 127: 1-14, 1993. PubMed: 8486275 Zabarovsky ER, Winberg GN. Improved methods and new vectors for creating libraries of cloned genes. Sov. Sci. Rev. 11: 1-65, 1992. |
| Shipped： | freeze-dried |