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购买须知
| 产品名称： | pESD 14.1.1 pESD14.1 |
|---|---|
| 商品货号： | TS204760 |
| Designations： | pESD 14.1.1 pESD14.1 |
| Species： | Homo sapiens, human |
| Depositors： | RA Phillips |
| Vector： | Construct size (kb): 4.300000190734863 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.200000047683716 Tissue: SV40-transformed fibroblast Gene product: esterase D/formylglutathione hydrolase( S-formylglutathione hydrolase, esterase D) ESD Alleles: A1, A2 |
| Insert Size (kb)： | 1.200 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): XhoI/PstI--1.8, 1.2, 1.1, 0.15; PstI--3.1, 1.28; XhoI--3.0, 1.35; BamHI--3.1, 0.65; HindIII--4.3. To obtain a full length (1.2 kb) fragment of the cDNA, cut with XhoI. To remove all vector sequences but 40 bp at the 3 end, cut with XhoI/PstI. The ApaI RFLP is distinct from the allelic isozymes because of different allele frequencies and because all 4 haplotypes of the 2 polymorphisms have been detected. |
| References： | Squire J, et al. Cloning of the esterase D gene: A polymorphic gene closely linked to the retinoblastoma locus on chromosome 13. Proc. Natl. Acad. Sci. USA 83: 6573-6577, 1986. PubMed: 3462714 |