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| 产品名称： | pRSVPAP |
|---|---|
| 商品货号： | TS204904 |
| Designations： | pRSVPAP |
| Depositors： | LA Culp |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli DH5alpha |
| Vector Information： | Size (kb): 10.1000003814697300 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pRc/RSV Intact vector size: 5.100 Type of vector: phagemid Vector end: HindIII Vector end: XbaI Cloning sites: HindIII SpeI BstXI NotI XbaI Polylinker sites: HindIII KpnI SacI BamHI SpeI XmaIII BstXI EcoRI PstI EcoRV BstXI NotI XhoI SphI NsiI XbaI Construction: pUC19, RSV, bovine growth hormone Host range: vertebrate cells; Escherichia coli Features (with orientation and position when available): marker(s): ampR, neoR, G418R promoter: RSV LTR replicon: pMB1, M13 terminator: bGH polyadenylation Cross references: Vector: pRSVPAP (phagemid) Promoters: Promoter RSV LTR Construction: pRc/RSV, pSV2Apap Marker(s):G418R,ampR,neoR Construct size (kb): 10.10000038146973 Features: marker(s): ampR, neoR, G418R promoter: RSV LTR replicon: pMB1, M13 terminator: bGH polyadenylation enhancer: RSV LTR |
| Applications： | contains sequence alkaline phosphatase, placental (Regan isozyme) expression vector reporter construct shuttle vector vector permitting production of single-stranded DNA vector permitting visual detection of activity in colonies |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--6.1, 4.0; HindIII--10.1; BglII--10.1; KpnI--5.0, 2.8, 2.3; XbaI--10.1. Transient expression experiments should be performed to examine the RSV promoter efficiency in different cell lines. The RSV promoter can be replaced by excising with a BglII/HindIII digest. Shuttle reporter plasmid permitting visual detection of activity by histochemical staining. Presence of alkaline phosphatase activity is used to follow cell lineage in culture or in situ. pRSVlacZII (ATCC 77129), pRSVPAP (TS204904) and pRSVADH (ATCC 77131) provide distinct color-staining reactions (aqua blue, red, and blue-black respectively) to permit simultaneous analysis of multiple lineages. The order of the major features in pRc/RSV is: RSV LTR - MCS - bovine growth hormone polyadenylation signal - M13 ori - SV40 early promoter - neoR - SV40 polyadenylation signal - pMB1 ori - bla. Constructed by cloning the HindIII/XbaI fragment from pSVApap into pRc/RSV. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Lin WC, Culp LA. Selectable plasmid vectors with alternative and ultrasensitive histochemical marker genes. BioTechniques 11: 344-351, 1991. PubMed: 1931036 Henthorn P, et al. Expression of a human placental alkaline phosphataes gene in transfected cells: use as a reporter for studies of gene expression. Proc. Natl. Acad. Sci. USA 85: 6342-6346, 1988. PubMed: 3413100 Lloyd A Culp, personal communication |
| Shipped： | freeze-dried |
| Shipping Information： | Distributed: freeze-dried |