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微信小章| 产品名称： | pKD136, pKD8.243A plasmids in E. coli |
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| 商品货号： | TS204928 |
| Designations： | pKD136, pKD8.243A plasmids in E. coli |
| Species： | Klebsiella pneumoniae (Schroeter) Trevisan |
| Depositors： | Purdue Univ. Res. Fndn., A Berry, Purdue Univ. Res. Fndn. |
| Applications： | produces protein 3-dehydroquinate synthase produces protein 3-dehydroshikimate dehydratase produces protein 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase produces protein protocatechuate decarboxylase produces protein transketolase |
| Vector： | Information on plasmid #1: pKD8.243A Size (kb): Unknown Marker: cmlR Insert component: Insert size (kb): unknown Genes: 3-dehydroshikimate dehydratase, aroZ, and protocatechuate decarboxylase, aroY Source: Klebsiella pneumoniae DNA: genomic DNA Vector information: Vector: unknown Markers: cmlR Information on plasmid #2: pKD136 Size (kb): 15.5 Marker: ampR Insert components (3 genes inserted): Gene 1: 3-dehydroquinate synthase, aroB Insert size (kb): 1.65 Source: Escherichia coli DNA: genomic DNA Insert ends: SphI Gene 2: transketolase, tkt Insert size (kb): 5.0 Source: Escherichia coli RB791 DNA: genomic DNA Insert ends: BamHI Gene 3: 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, aroF Insert size (kb): 2.4 Source: Escherichia coli DNA: genomic DNA Insert ends: EcoRI linkers Vector information: Vector: pBR325 Vector size (kb): 5.7 Marker: ampR |
| Media： | ATCC Medium 1065 plus ampicillin (50 mcg/ml) plus chloramphenicol (50 mcg/ml)
ATCC Medium 1065:
Tryptone (Difco 0123), 10.0 g
Yeast Extract (Difco 0127), 5.0 g
NaCl, 10.0 g
Distilled water, 1.0 L
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| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Freeze-dried plasmids in E. coli |
| Comments： | Restriction digests of the plasmids in this strain give the following sizes (kb): BamHI--10.0, 5.0, 4.8, 3.4, 1.1, 0.3; EcoRI--9.2, 8.8, 4.0, 2.4; SphI--18.0, 4.4, 1.8. This strain supports a portion of the biocatalytic conversion of D-glucose into adipate (a reagent in the production of nylon). It converts D-glucose into catechol. Colonies turn brown as catechol accumulates. The tkt CDS can be recovered as a 2.4 kb EcoRI fragment. The aroF CDS can be recovered as a 5.0 kb BamHI fragment. The aroB CDS can be recovered as a 1.65 kb SphI fragment. |
| References： | Draths KM, Frost JW. Genomic direction of synthesis during plasmid-based biocatalysis. J. Am. Chem. Soc. 112: 9630-9632, 1990. Draths KM, Frost JW. Synthesis using plasmid-based biocatalysis: plasmid assembly and 3-deoxy-D-arabino-heptulosonate production. J. Am. Chem. Soc. 112: 1657-1659, 1990. Draths KM, Frost JW. Environmentally compatible synthesis of adipic acid from D-glucose. J. Am. Chem. Soc. 116: 399-400, 1994. Frost JW. Enhanced production of common aromatic pathway compounds. US Patent 5,168,056 dated Dec 1 1992 Pittard J, Wallace BJ. Distribution and function of genes concerned with aromatic biosynthesis in Escherichia coli. J. Bacteriol. 91: 1494-1508, 1966. PubMed: 5326114 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |