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| 产品名称： | pEFZ10 |
|---|---|
| 商品货号： | TS204933 |
| Designations： | pEFZ10 |
| Species： | Homo sapiens, human |
| Depositors： | RL White, Y Nakamura |
| Vector： | Construct size (kb): 6.5 |
| Insert： | DNA: genomic Insert lengths(kb): 3.799999952316284 Gene product: DNA Segment, single copy D18S20 |
| Insert Size (kb)： | 3.800 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): HincII--3.05, 1.9, 1.25; BamHI--4.4, 2.1; EcoRI--3.1, 1.9, 1.5; HindIII--6.5; PvuII--2.35, 2.2, 1.95. This probe was also known as D18S20. This probe should be prehybridized with total human DNA (50 ug/ml) for 2-3 hours at 42C. |
| References： | Nakamura Y, et al. Variable number of tandem repeat (VNTR) markers for human gene mapping. Science 235: 1616-1622, 1987. PubMed: 3029872 Krapcho K, et al. Isolation of mapping of a polymorphic DNA sequence pEFZ10 on chromosome 18 D18S22. Nucleic Acids Res. 16: 1227, 1988. PubMed: 2894012 Peter J OConnell, personal communication |