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| 产品名称： | pB1-14 |
|---|---|
| 商品货号： | TS204979 |
| Designations： | pB1-14 |
| Species： | Mus musculus, mouse |
| Depositors： | U Storb |
| Vector： | Construct size (kb): 20.0 |
| Insert： | DNA: genomic Insert lengths(kb): 15.60000038146973 Tissue: myeloma MOPC-21 cell line Gene product: immunoglobulin kappa chain complex Igk |
| Insert Size (kb)： | 15.600 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | BamHI digestion gives a 2.0 kb fragment containing the VL exon, a 5.3 kb fragment containing the V MOPC-21, J2-J5, and Ckappa exons, a larger fragment about 7.5 kb, and 3 smaller fragments. Restriction digests of the clone give the following sizes (kb): EcoRI--16.0, 4.4; AvaI--13.0, 4.0, 3.7; BglI/XhoI--15.0, 2.4, 2.15; EcoRV--8.8, 6.2, 3.7, 1.0. A BglI/XhoI double digest gives a 14.8 kb fragment containing some pBR322 sequences plus all the exons, and smaller XhoI/BglI and BglI fragments. The insert is an EcoRI fragment from the MOPC-21 kappa gene which was cloned into Charon 4A (M21B1). The insert includes 5 flanking regions, VL, V MOPC-21, J2-J5, Ckappa, and approximately 8.2 kb 3 flanking sequence. |
| Classification： | Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References： | Brinster RL, et al. Expression of a microinjected immunoglobulin gene in the spleen of transgenic mice. Nature 306: 332-336, 1983. PubMed: 6417544 |