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微信小章| 产品名称： | pSE1000 |
|---|---|
| 商品货号： | TS205027 |
| Designations： | pSE1000 |
| GenBank Number： | X61622 |
| Species： | Homo sapiens, human |
| Depositors： | SJ Elledge |
| Applications： | in another host, produces protein cyclin-dependent kinase 2 |
| Vector： | Construct size (kb): 9.800000190734863 |
| Insert： | DNA: cDNA Insert lengths(kb): 2.0 Tissue: lymphocytes, peripheral, EBV-transformed Gene product: cyclin-dependent kinase 2 CDK2 Target Gene: cyclin-dependent kinase 2 |
| Insert Size (kb)： | 2.0 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): XhoI--7.7, 2.0; PvuII--9.8; BamHI--9.8; EcoRI--7.6, 1.4, 0.75; SmaI--6.6, 3.2. The sequenced portion of the insert predicts the following restriction sites (approximate kb from the 5 end): PvuII--0.33, 0.55; SacI--0.51; BamHI--1.26; EcoRI--1.38; SmaI--1.46. Contains the complete coding region (nt 1-897 of the reported sequence). The sequence record includes the first 1476 nt from the ATG initiation codon; the remainder of the 3 untranslated region has not been reported. Detects a transcript of 2.1 kb in human cells. The vector is the plasmid derivative of lambdaYES-R. The insert is oriented such that transcription is regulated by the GAL1 promoter when transformed into S. cerevisiae. The construct was identified by complementation of the cdc28-4 mutation in yeast strain Y10. |
| References： | Elledge SJ, Spottswood MR. A new human p34 protein kinase, CDK2, identified by complementation of a cdc28 mutation in Saccharomyces cerevisiae, is a homolog of Xenopus Eg1. EMBO J. 10: 2653-2659, 1991. PubMed: 1714386 Elledge SJ, et al. lambdaYES: a multifunctional cDNA expression vector for the isolation of genes by complementation of yeast and Escherichia coli mutations. Proc. Natl. Acad. Sci. USA 88: 1731-1735, 1991. PubMed: 1848010 Elledge SJ, et al. CDK2 encodes a 33-kDa cyclin A-associated protein kinase and is expressed before CDC2 in the cell cycle. Proc. Natl. Acad. Sci. USA 89: 2907-2911, 1992. PubMed: 1372993 |