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| 产品名称： | pBendBlue |
|---|---|
| 商品货号： | TS205033 |
| Designations： | pBendBlue |
| Depositors： | GJ Wistow, SJ Sperbeck |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 3.0999999046325680 Vector: pBendBlue (phagemid) Construction: pBluescriptII SK, pBend2 polylinker Marker(s):ampR Construct size (kb): 3.099999904632568 Features: marker(s): ampR replicon: f1 replicon: pMB1 reporter group: lacZ MCS: KpnI...SacI |
| Applications： | vector containing primer sites useful for sequencing vector for studying DNA-protein interactions vector permitting visual detection of recombinants beta-galactosidase beta-D-galactosidase |
| Comments： | Restriction digests of the clone give the following sizes (kb): SalI--3.1; XbaI--3.1; EcoRI/HindIII--2.9, 0.28. Target DNA binding sites can be cloned into the vector and an array of fragments can be generated (by restriction digestion with different enzymes) that are equal in length, but differ in the position of the DNA binding site along the fragment. The reduced mobility of different protein-fragment complexes can then by analyzed by gel electrophoresis to determine the degree of bending induced by protein binding. Cloning vector with lacZ color detection designed for studying the amount of bending induced at a DNA binding site due to DNA-protein interactions. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Sperbeck SJ, Wistow GJ. pBendBlue: Modification of the pBend System for color selectability. BioTechniques 24: 66-68, 1998. PubMed: 9454955 |
| Shipped： | freeze-dried |