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| 产品名称： | pMOB4 |
|---|---|
| 商品货号： | TS205073 |
| Designations： | pMOB4 |
| Species： | pCU1 |
| Depositors： | HP Schweizer |
| Vector： | Construct size (kb): 5.800000190734863 |
| Insert： | DNA: genomic Insert lengths(kb): 2.700000047683716 Gene product: (IncN) origin of transfer replication oriT |
| Insert Size (kb)： | 2.700 |
| Media： | ATCC Medium 1065 (see below) plus kanamycin (50.0 mcg/ml) plus chloramphenicol (25.0 mcg/ml) ATCC Medium 1065: Tryptone (Difco 0123), 10.0 g Yeast Extract (Difco 0127), 5.0 g NaCl, 10.0 g Distilled water, 1.0 L
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| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): XbaI--5.8; NotI--3.4, 2.4; EcoRI/HindIII--2.8, 1.5, 0.55 (doublet), 0.2. Available for research purposes only. Plasmid containing an oriT/cmlR mobilization cassette that can be inserted into a general purpose vector (ATCC 77278, 77279) for conjugal transfer of cloned inserts into the chromosome of P. aeruginosa. Constructed by deleting the 2.5 kb XbaI fragment from pMOB3 (ATCC 77282). The NotI digest excises a fragment of approximately 3.0 kb that includes short segments of the pHSS21 sequence. The NotI cassette includes these features: pHSS21 sequence - EcoRI - oriT - PstI - BamHI - HindIII - cmlR - HindIII - BamHI - XbaI - sacR fragment/SalI - PstI - SphI - HindIII - pHSS21 sequence. |
| References： | Schweizer HP. Allelic exchange in Pseudomonas aeruginosa using novel ColE1-type vectors and a family of cassettes containing a portable oriT and the counter-selectable Bacillus subtilis sacB marker. Mol. Microbiol. 6: 1195-1204, 1992. PubMed: 1588818 |