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| 产品名称： | pMCT108.2 |
|---|---|
| 商品货号： | TS205074 |
| Designations： | pMCT108.2 |
| Species： | Homo sapiens, human |
| Depositors： | RL White, Y Nakamura |
| Applications： | Codominant segregation of the MspI RFLP was observed in 48 three-generation families. |
| Vector： | Construct size (kb): 5.900000095367432 |
| Insert： | DNA: genomic Insert lengths(kb): 3.200000047683716 Gene product: DNA Segment, single copy D18S24 Alleles: B1, B2, A1, A2 |
| Insert Size (kb)： | 3.200 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--5.7, 0.66; HindIII--6.0; PstI--5.4, 0.37; BamHI--6.0; EcoRI/HindIII--2.95, 2.5, 0.62; MspI--2.3, 1.2, 0.45, 0.39, 0.29, 0.25; consistent with deposited DNA. Codominant segregation of the MspI RFLP was observed in 48 three-generation families. The insert appears to be chimeric, with the large MspI fragment detecting the polymorphism on chromosome 18. A fragment adjacent to the EcoRI side of the polylinker has been mapped to chromosome 9. Enzyme(s) not detecting polymorphism: RsaI, PstI, PvuII and BglII. |
| References： | Carlson M, et al. Isolation and mapping of a polymorphic DNA sequence (pMCT108.2) on chromosome 18 D18S24. Nucleic Acids Res. 16: 4188, 1988. PubMed: 2453850 Lia KM, et al. pMCT108.2 is a chimaeric clone which physically maps to human chromosome bands 9q33 (D9S203) and 18q21.1 (D18S24). Hum. Mol. Genet. 2: 332, 1993. PubMed: 8499927 |