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| 产品名称： | pZilch-NLS |
|---|---|
| 商品货号： | TS205130 |
| Designations： | pZilch-NLS |
| Depositors： | C Hovens |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 6.1059999465942380 Vector: pZilch-NLS (phagemid) Promoters: Promoter CMV Construction: pCATCH-NLS Marker(s):ampR Construct size (kb): 6.105999946594238 Features: marker(s): ampR promoter: CMV promoter for in vitro transcription: SP6 promoter for in vitro transcription: T7 replicon: M13 replicon: SV40 ori replicon: pMB1 replicon: polyoma MCS: BamHI...XhoI MCS: BglII...XmaI signal peptide coding region: SV40 NLS terminator: SV40 coding sequence: mutant pheS gene epitope tag: FLAG |
| Applications： | encodes removable tag for protein isolation expression vector vector for assessing nuclear localization signals vector permitting construction of fusion proteins vector permitting positive selection for inserts vector permitting production of single-stranded DNA |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--6.1; EcoRI--6.1. The vector was contructed by inserting a 1185 bp SmaI/BamHI fragment from pKSS encompassing the mutant pheS allele into the pCATCH-NLS and modified further to generate polylinker and to incorporate Kozak sequence and initiation ATG codon. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Schneider S, et al. An epitope tagged mammalian/prokaryotic expression vector with positive selection of cloned inserts. Gene 197: 337-341, 1997. PubMed: 9332383 |
| Shipped： | freeze-dried |