微信小陈
微信小章
产品简介
购买须知
| 产品名称： | YRp15-leu2delta1 YRp15/leu2-delta1, p307 |
|---|---|
| 商品货号： | TS205172 |
| Designations： | YRp15-leu2delta1 YRp15/leu2-delta1, p307 |
| Depositors： | P Hieter |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 7.6999998092651370 Vector: YRp15-leu2delta1 (plasmid) Construction: YRp15 Marker(s):URA3,ampR,SUP11 Construct size (kb): 7.699999809265137 Features: marker(s): ampR, URA3, SUP11 replicon: pMB1, ARS1 |
| Applications： | vector for other uses |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI/ClaI--6.1, 1.6; SalI--5.9, 1.8; EcoRI/HindIII--6.2, 1.5; HindIII--7.7. A marker addition/transplacement vector. Non-integrants grow poorly selecting for Ura3+ (pinpoints at 5 days) while integrants grow well because the SUP11 copy number is maintained at one. If there are no integrants on a transformation plate, streak onto -URA plates and pick papillae. A 0.6 kb EcoRI/ClaI fragment (blunt-ended) was deleted from a 2.2 kb genomic SalI/XhoI fragment having a SalI linker. The SalI fragment was cloned into YRp15. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Sikorski RS, Hieter P. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122: 19-27, 1989. PubMed: 2659436 Philip Hieter, personal communication |
| Shipped： | freeze-dried |