| 产品名称: | LORIC |
|---|---|
| 商品货号: | TS205209 |
| Designations: | LORIC |
| Depositors: | PF Little, SJ Cross |
| Biosafety Level: | 1 |
| Vector Information: | Size (kb): 6.2699999809265140 Vector: LORIC (cosmid) Promoters: Promoter none Construction: Picos, pBRneo, lambda ori Marker(s):kanR Construct size (kb): 6.269999980926514 Features: marker(s): kanR promoter: none replicon: lambda terminator: none enhancer: none |
| Applications: | vector for constructing genomic libraries vector for other uses |
| Comments: | IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. Cosmid vector which facilitates restriction enzyme mapping. Derived from phage lambda origin of replication. |
| Media: | ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin |
| Growth Conditions: | Temperature: 30.0°C |
| References: | Little PF, Cross SH. A cosmid vector that facilitates restriction enzyme mapping. Proc. Natl. Acad. Sci. USA 82: 3159-3163, 1985. PubMed: 2987911 |
| Shipped: | freeze-dried |