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| 产品名称： | pSV2-hph |
|---|---|
| 商品货号： | TS205394 |
| Designations： | pSV2-hph |
| Depositors： | P Berg, M Dieckmann |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 5.5000000000000000 Vector: pSV2-hph (plasmid) Promoters: Promoter SV40 early Marker(s):ampR,hygR Construct size (kb): 5.5 Features: marker(s): ampR, hygR promoter: SV40 early replicon: pMB1 terminator: SV40 enhancer: SV40 early |
| Applications： | co-transfection marker aminocyclitol phoshotransferase hygromycin B phosphotransferase expression vector shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): HindIII/BglII--3.87, 1.34, 0.69; PvuII/PstI--3.66, 1.84; PvuII--uncut. An Escherichia coli/mammalian cell shuttle vector. Consists of the SV40 promoter (SV nt 270-5171), a HindIII linker, the hph gene (1347 bp), SV40 splice site (SV nt 4710-4100), SV40 polyadenylation site (SV nt 4362-2067), pML (inserted at the BamHI site with HindIII-EcoRI-amp upstream of the SV40 promoter). |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Gritz L, Davies J. Plasmid-encoded hygromycin B resistance: the sequence of hygromycin B phosphotransferase gene and its expression in Escherichia coli and Saccharomyces cerevisiae. Gene 25: 179-188, 1983. PubMed: 6319235 Montrose-Rafizadeh C, et al. High potency antagonists of the pancreatic glucagon-like peptide-1 receptor. J. Biol. Chem. 272: 21201-21206, 1997. PubMed: 9261127 Paul Berg, personal communication |
| Shipped： | freeze-dried |
| Shipping Information： | Distributed: freeze-dried |