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微信小章| 产品名称： | Escherichia coli (Migula) Castellani and Chalmers |
|---|---|
| 商品货号： | TS205431 |
| Classification： | Enterobacteriaceae, Escherichia |
| Strain Designations： | JMB9 (pRK248cIts) |
| Application： | transformation host |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen |
| Preceptrol®： | no |
| Genotype： | F- deltatrpEB9 supE44 lacY1 thr-1 leuB6 (pRK248cIts) thi-1 fhuA21 lambda- |
| Type Strain： | no |
| Comments： | Restriction digests of pRK248cIts give the following sizes (kb): EcoRI--11.0; SalI--11.0; NcoI--10.5, 0.5; XbaI--uncut; SphI--11.0. The plasmid confers tetracycline resistance. Contains the plasmid pRK248cIts encoding a temperature-sensitive lambda repressor. Because this plasmid is compatible with ColE1 replicons, the strain can serve as a host for plasmids with expression to be thermally regulated from a lambda promoter. |
| Medium： | ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline |
| Growth Conditions： | Temperature: 30.0°C |
| Name of Depositor： | H Bernard |
| References： | Bernard HU, et al. Construction of plasmid cloning vehicles that promote gene expression from the bacteriophage lambda pL promoter. Gene 5: 59-76, 1979. PubMed: 372049 Thomas CM, et al. Regions of broad-host-range plasmid RK2 whihc are essential for replication and maintenance. J. Bacteriol. 141: 213-222, 1980. PubMed: 6243621 Kahn M, et al. Plasmid cloning vehicles derived from plasmids ColE1, F, R6K, and RK2. Methods Enzymol. 68: 268-280, 1979. PubMed: 232215 Bernard HU, Helinski DR. Use of the lambda phage promoter PL to promote gene expression in hybrid plasmid cloning vehicles. Methods Enzymol. 68: 482-492, 1979. PubMed: 232223 Hans-Ulrich Bernard, personal communication Douglas P Cerretti, personal communication |