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微信小章| 产品名称： | MES-OV |
|---|---|
| 商品货号： | TS205478 |
| Organism： | Homo sapiens, human |
| Tissue： | ovary |
| Product Format： | frozen 1.0 mL |
| Morphology： | epithelial-like |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | ovarian serous cystadenocarcinoma, stage III, grade 2 |
| Age： | 53 years |
| Gender： | female |
| Applications： | This is an excellent in vitro and in vivo model of ovarian cancer. Useful for gene expression profiling.
Ref  Schaner ME, et al. Gene expression patterns in ovarian carcinomas. Mol. Biol. Cell 14: 4376-4386, 2003. PubMed: 12960427 |
| Storage Conditions： | liquid nitrogen vapor phase |
| Images： |  |
| Derivation： | CRL-3272 cells were isolated from the ascites fluid from a 53 year-old female patient with stage III ovarian cystadenocarcinoma. |
| Clinical Data： | 53 years old female |
| Cellular Products： | collagen, lumican, matrix metalloproteinase 2, SPARC (secreted protein acidic and rich in cysteine), AXL receptor tyrosine kinase |
| Comments： | CRL-3272 cells were isolated from the ascites fluid from a 53 year-old female patient with Stage III ovarian cystadenocarcinoma. Clonogenic assays performed at the time of isolation indicated that this cell line is quite sensitive to vinca alkaloids (vinblastine) (Deposit Form Information). Based on gene expression profiling, MES-OV manifests some mesenchymal features, with high expression of collagens (type VI, alpha 3, type I, alpha 2, type III, and alpha 1), lumican, matrix metalloproteinase 2, and SPARC. This cell line lacks many of the distinct molecular characteristics of epithelial cells, typically seen in ovarian neoplasms, although expression of MUC1 and mesothelin was notable (PubMed: 12960427). This metastatic cell line also highly expresses AXL receptor tyrosine kinase protein (PubMed: 20858715). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated McCoys 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
xa0xa0 Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
xa0 Subcultivation Ratio: 1:4 to 1:10 is recommended. Medium Renewal: 2 to 3 times a week |
| Cryopreservation： | Freeze Medium: Complete growth medium, 90%; DMSO, 10% Storage Temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| Volume： | 1.0 mL |
| STR Profile： | Amelogenin: X CSF1PO: 10 D13S317: 9,11 D16S539: 11,12 D5S818: 13 D7S820: 11 TH01: 6,9 TPOX: 8,11 vWA: 17 |
| Population Doubling Time： | approximately 22 hours (personal communication) |
| Name of Depositor： | B Sikic, Stanford University |
| Year of Origin： | 1980 |
| References： | Wang YC, et al. Regional activation of chromosomal arm 7q with and without gene amplification in taxane-selected human ovarian cancer cell lines. Genes Chromosomes Cancer 45: 365-374, 2006. PubMed: 16382445 Rankin EB, et al. AXL is an essential factor and therapeutic target for metastatic ovarian cancer. Cancer Res. 70(19): 7570-7579, 2010. PubMed: 20858715 Schaner ME, et al. Gene expression patterns in ovarian carcinomas. Mol. Biol. Cell 14: 4376-4386, 2003. PubMed: 12960427 |