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微信小章| 产品名称： | Aspergillus awamori Nakazawa |
|---|---|
| 商品货号： | TS205834 |
| Strain Designations： | NRRL 3112 |
| Application： | Produces amylase, alpha 1A; salivary Amylase, salivary, alpha-1A
Produces ethyl alcohol ethanol
Produces glucamylase
Produces glucoamylase
Produces glucuronidase, beta beta-glucuronidase
Transformation host
Produces extracellular alpha-amylase
Produces endogenous beta-glucuronidase |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | freeze-dried |
| Storage Conditions： | Frozen: -80°C or colder Freeze-Dried: 2°C to 8°C Live Culture: See Propagation Section |
| Type Strain： | no |
| Preceptrol®： | no |
| Comments： | Cloning of glucoamylase gene
Carries dsRNA mycovirus |
| Medium： | ATCC® Medium 336: Potato dextrose agar (PDA) ATCC® Medium 312: Czapeks agar ATCC® Medium 325: Malt extract agar (Blakeslees formula) |
| Growth Conditions： | Temperature: 24°C to 26°C Atmosphere: Typical aerobic |
| Sequenced Data： |
18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence GGTTTCCGTAGGTGAACCTGCGGAAGGATCATTACCGAGTGCGGGTCCTTTGGGCCCAACCTCCCATCCGTGTCTATTGTACCCTGTTGCTTCGGCGGGCCCGCCGCTTGTCGGCCGCCGGGGGGGCGCCTCTGCCCCCCGGGCCCGTGCCCGCCGGAGACCCCAACACGAACACTGTCTGAAAGCGTGCAGTCTGAGTTGATTGAATGCAATCAGTTAAAACTTTCAACAATGGATCTCTTGGTTCCGGCATCGATGAAGAACGCAGCGAAATGCGATAACTAATGTGAATTGCAGAATTCAGTGAATCATCGAGTCTTTGAACGCACATTGCGCCCCCTGGTATTCCGGGGGGCATGCCTGTCCGAGCGTCATTGCTGCCCTCAAGCCCGGCTTGTGTGTTGGGTCGCCGTCCCCCTCTCCGGGGGGACGGGCCCGAAAGGCAGCGGCGGCACCGCGTCCGATCCTCGAGCGTATGGGGCTTTGTCACATGCTCTGTAGGATTGGCCGGCGCCTGCCGACGTTTTCCAACCATTCTTTCCAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAAGCATATCAATAA D1D2 region of the 28S ribosomal RNA gene ATATCAATAAGCGGAGGAAAAGAAACCAACCGGGATTGCCTCAGTAACGGCGAGTGAAGCGGCAAGAGCTCAAATTTGAAAGCTGGCTCCTTCGGAGTCCGCATTGTAATTTGCAGAGGATGCTTTGGGTGCGGCCCCCGTCTAAGTGCCCTGGAACGGGCCGTCAGAGAGGGTGAGAATCCCGTCTTGGGCGGGGTGTCCGTGCCCGTGTAAAGCTCCTTCGACGAGTCGAGTTGTTTGGGAATGCAGCTCTAAATGGGTGGTAAATTTCATCTAAAGCTAAATACTGGCCGGAGACCGATAGCGCACAAGTAGAGTGATCGAAAGATGAAAAGCACTTTGAAAAGAGAGTTAAACAGCACGTGAAATTGTTGAAAGGGAAGCGCTTGCGACCAGACTCGCCCGCGGGGTTCAGCCGGCATTCGTGCCGGTGTACTTCCCCGTGGGCGGGCCAGCGTCGGTTTGGGCGGCCGGTCAAAGGCCCCTGGAATGTAGTGCCCTCCGGGGCACCTTATAGCCAGGGGTGCAATGCGGCCAGCCTGGACCGAGGAACGCGCTTCGGCACGGACGCTGGCATAATGGTCGTAAACGAC beta-tubulin gene GGAGAGCGTCGTTGGTTGGCATCTCTTTTGCTCGGGACCCCACCGGTTCTTCGACCAACTCATTCTTGTGCTAACTGCATGTCTTCTTCGCTTCATAGGTTCACCTCCAAACCGGCCAGTGTGTAAGTGCCAATATATGCTTCGGATGATTGCCCCCAAGGGTCTTGATTGGTGTTTGGTGGACTAAACAATATATCATGGTGGTTAGGGTAACCAAATTGGTGCTGCTTTCTGGTACGTATACAACTGCCATTGGATTGGGGATGGAACATCGTCTCTTAGGCTATCTCAGCTTGAGTTCAGATGTTGTCCATTAGGTACATGCTATCGGTCTAAGAACACGTCTAACAATTCAACAGGCAGACCATCTCTGGCGAGCACGGCCTTGACGGCTCCGGTGTGTAAGTGCAACTTTTTCACACCTCTCAATTGGTCAACAATGGGCAAAGGGTTGGGTCTTCTGACACGCAGGATAGTTACAATGGCACCTCCGACCTCCAGCTGGAGCGCATGAACGTCTACTTCAACGAGGTGAGATCCATCGGACCTTGGCTTTTTCACGACAATATCATCAATGTCCTAATCACTTCAGCAGGCTAGCGGTAACAAGTATGTTCCTCGTGCCGTCCTCGTCGACCTCGAGCCCGGTACCATGGACGCCGTCCGTGCCGGTCCTTTCGGCCAGCTCTTCCGCCCCGACAACTTCGTCTTCGGCCAGTCCGGTGCTGGTAACAACTGG |
| Name of Depositor： | NRRL |
| Chain of Custody： | ATCC |
| Isolation： | Bran |
| Cross References： | Nucleotide (GenBank) : Y15784 Aspergillus awamori gdhA gene. Nucleotide (GenBank) : Y12504 A.awamori bip gene and promoter sequence. Nucleotide (GenBank) : X53576 Aspergillus niger trpC gene for glutamine amidotransferase, |
| References： | Altosaar I, Bhella RS. Purification and some properties of the extracellular alpha-amylase from Aspergillus awamori. Can. J. Microbiol. 31: 149-153, 1985. Andreasan AA, et al. Production of glucoamylase for conversion of grain mashes in the production of grain spirits. US Patent 3,988,204 dated Oct 26 1976 Bhella RS, Altosaar I. Production of multiple forms of glucoamylase in Aspergillus awamori. Biochem. Cell Biol. 65: 762-765, 1987. PubMed: 3124870 . . DECHEMA Biotechnol. Conf. 3A: 325-328, 1989. Dworschack RG, Nelson CA. Production of glucoamylase. US Patent 3,660,236 dated May 2 1972 Gottschalk TE, et al. Detection of endogenous beta-glucuronidase activity in Aspergillus niger. Appl. Microbiol. Biotechnol. 45: 240-244, 1996. PubMed: 8920195 . . J. Sci. Ind. Res. 14: 247-250, 1971. Kumar Soni S, et al. Studies on glucoamylase produced from Aspergillus awamori (NRRL-3112) and their effect on saccharification of potato starch. Indian J. Exp. Biol. 33: 957-961, 1995. PubMed: 8714078 Nunberg JH, et al. Molecular cloning and characterization of the glucoamylase gene of Aspergillus awamori. Mol. Cell. Biol. 4: 2306-2315, 1984. PubMed: 6440004 Smiley KL. Process for obtaining amyloglucosidase. US Patent 3,301,768 dated Jan 31 1967 Van Lanen JM, Smith MB. Process for producing glucamylase and an alcohol product. US Patent 3,418,211 dated Dec 24 1968 Varga J, et al. Double-stranded RNA mycoviruses in section Nigri of the Aspergillus genus. Can. J. Microbiol. 40: 325-329, 1994. PubMed: 8039055 Martinez-Culebras PV, et al. Molecular characterization of the black Aspergillus isolates responsible for ochratoxin A contamination in grapes and wine in relation to taxonomy of Aspergillus section Nigri. Int. J. Food Microbiol. 132: 33-41, 2009. PubMed: 19401261 Hijarrubia MJ, et al. Characterization of the bip gene of Aspergillus awamori encoding a protein with an HDEL retention signal homlogous to the mammalian BiP involved in polypeptide secretion. Curr. Genet. 32: 139-146, 1997. PubMed: 9294262 |