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微信小章| 产品名称： | Filobasidiella neoformans Kwon-Chung, teleomorph |
|---|---|
| 商品货号： | TS206204 |
| Deposited As： | Cryptococcus neoformans (Sanfelice) Vuillemin |
| Strain Designations： | NIH B-3501 CBS 6900, CBS 7697, CBS 7817, CCRC 20532 |
| Application： | Biomedical Research and Development Material
assay of eumelanin pigments Ref
  Williamson PR, et al. Melanin biosynthesis in Cryptococcus neoformans. J. Bacteriol. 180: 1570-1572, 1998. PubMed: 9515929xa0RefLiu L, et al. Catecholamine oxidative products, but not melanin, are produced by Cryptococcus neoformans during neuropathogenesis in mice. Infect. Immun. 67: 108-112, 1999. PubMed: 9864203produces monophenol monooxygenase phenol oxidase, phenoloxidase Ref
Ikeda R, et al. Characterization of a phenol oxidase from Cryptococcus neoformans var. neoformans. Microbiol. Immunol. 37: 759-764, 1993. PubMed: 8289682regulation of phenol oxidase and capsular polysaccharide Ref Jacobson ES, Compton GM. Discordant regulation of phenoloxidase and capsular polysaccharide in Cryptococcus neoformans. J. Med. Vet. Mycol. 34: 289-291, 1996. PubMed: 8873890 |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | freeze-dried |
| Storage Conditions： | Frozen: -80°C or colder Freeze-Dried: 2°C to 8°C Live Culture: See Propagation Section |
| Type Strain： | no |
| Antigenic Properties： | serotype D
RefSchmeding KA, et al. Sexual compatibility between serotypes of Filobasidiella neoformans (Cryptococcus neoformans). Curr. Microbiol. 5: 133-138, 1981. , RefKwon-Chung KJ. Morphogenesis of Filobasidiella neoformans, the sexual state of Cryptococcus neoformans. Mycologia 68: 821-833, 1976. PubMed: 790172 |
| Genotype： | VNIV |
| Preceptrol®： | no |
| Genome Sequenced Strain： | Yes |
| Mating Type： | alpha
RefKwon-Chung KJ. Nuclear genotypes of spore chains in Filobasidiella neoformans (Cryptococcus neoformans). Mycologia 72: 418-422, 1980. PubMed: 6991936 |
| Comments： | Designated as type strain of Cryptococcus deneoformans Hagen et Boekhout by Hagen et al. (2015).
Genome sequencing strain (The University of Oklahoma, USA).
Smooth colony type Ref
Kwon-Chung KJ. Nuclear genotypes of spore chains in Filobasidiella neoformans (Cryptococcus neoformans). Mycologia 72: 418-422, 1980. PubMed: 6991936 |
| Morphology： | After 3 days on YM medium at 25°C, colony is cream-colored, mucoid. Cells are globose, single. No hyphae observed. |
| Medium： | ATCC® Medium 28: Emmons modification of Sabourauds agar ATCC® Medium 200: YM agar or YM broth ATCC® Medium 1245: YEPD |
| Growth Conditions： | Temperature: 25°C to 30°C Atmosphere: Typical aerobic |
| Sequenced Data： |
18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 26S ribosomal RNA gene, partial sequence
GGTTTCCGTAGGTGAACCTGCGGAAGGATCAGTAGAGAATATTGGACTTTGGTCCATTTATCTACCCATCTACACCTGTGAACTGTTTATGTGCTTCGGCACGTTTTACACAAACTTCTAAATGTAATGAATGTAATCATATTATAACAATAATAAAACTTTCAACAACGGATCTCTTGGCTTCCACATCGATGAAGAACGCAGCGAAATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAGTCTTTGAACGCAACTTGCGCCCTTTGGTATTCCGAAGGGCATGCCTGTTTGAGAGTCATGAAAATCTCAATCCCTCGGGTTTTATTACCTGTTGGACTTGGATTTGGGTGTTTGCCGCGACCTGCAAAGGACGTCGGCTCGCCTTAAATGTGTTAGTGGGAAGGTGATTACCTGTCAGCCCGGCGTAATAAGTTTCGCTGGGCCTATGGGGTAGTCTTCGGCTTGCTGATAACAACCATCTCTTTTTGTTTGACCTCAAATCAGGTAGGGCTACCCGCTGAACTTAAGCATATCAATAA D1D2 region of the 26S ribosomal RNA gene ATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTAGTAACGGCGAGTGAACCGGGAAGAGCTCAAATTTGAAATCTGGCGTCCTCCGGGCGTCCGAGTTGTAATCTACAGAAACGTTTTCCGTGCTGGACCGTGTCTAAGTCCCTTGGAATAGGGTATCAAAGAGGGTGACAATCCCGTACTTGACACGATCACCAGTGCTCTGTGATACGTTTTCTACGAGTCGCGTTACTTGGGAGTGTAGCGCAAAATGGGTGGTAAACTCCATCTAAAGCTAAATATTGGTGGAAGACCGATAGCGAACAAGTACCGTGAGGGAAAGATGAAAAGCACTTTGGAAAGAGAGTTAAACAGTACGTGAAATTGTTGAAAGGGAAACGATTGAAGTCAGTCGTGTCTATTGGGTTCAGCCAGTTCTGCTGGTGTATTCCCTTTAGACGGGTCAACATCAGTTCTGATCGGTGGATAAGGGCTGGAGGAATGTGGCACTCTTCGGGGTGTGTTATAGCCTCCTGTCGCATACACTGGTTGGGACTGAGGAATGCAGCTCGCCTTTATGGCCGGGGTTCGCCCACGTTCGAGCTTAGGATGTTGACAAAATGGCTTTAAACGAC 26S ribosomal RNA gene and intergenic spacer (IGS), partial sequence ATGGGAACGGGGTGCTGTAAGTGGTAGAGTAGCCTTGTTGCTACGATCCACTGAGGCTAAGCCCTTGTTCTATAGATTTGTCTCTAACATGTTGGGTCTCGGGGGGCTTCCTCTAGAGACTTGGATGTAAGGGGCTTTACGCATTCACATAATATTAGTTGGGGACTGAGGGGGGTGTGTGCTGTGCGAGCCCGGCTGATAATTGTTTTAATTGTTAGCTTGGATTGCACACACAGTCTCATCAGTCTTCAGCTTGGGTTATCGATATTCTGTAAGTCCAAACTTATCCATTCACATTCACTGTAGTGTAGGTTGCAGAGAGGACTTGGAAAGTTCTCTCTCTGCACTGGGCATACTACTGATTTTATGGTCATGGGGGACTTGGGAGCTGGTGCTTGTGTCGCATAAGGTAGAGTTGATGGTTGGACAGATAGATAAGCATTTTTATGTCGCGGGGGACTTGGGGTAAGACGCCTTGCAGCAAGTAAGAGAGTCAAACAGTAGTTACTTGTTCTAGTCGTGGGGGACTTTGGTAGTGGTAGTGAGCCGGAATGAGAGATGGATAAATGCAGTATGGATGGTGACAGGAGGAGCAGCAGAGCGTATGCAGTGGATATGTATATAGTTGAGAAGGGATGGACAGTAGAACGTTTGAACGTTGAGGAATAATTGACCCAAGGGTGATTCTAATAGACTTGTCAAGAAAAAGTTAAGTTGAATCAAGCTTGGTCAAGCAAAGTCTAGAAAAGATTTACAATCACCAAGAATTGCCCGAGTCGCGGGGGGCTTGTCACTTCTCTTGCTCATTGTGGGTCCAGTCT glycerol-3-phosphate dehydrogenase 1 (GPD1) gene, partial cds CAACGGTTTCGGTACGTAATCAATCCTTCTCTTCCCGTTGCTTCATATCCCACCGATGTGACTTCCAATATTGTGCGCTGGCTGTTCTTGAGTTATGGTAGCTAATAGCTATGTGATAGGTCGTATTGGTCGAATTGTTCTCAGGTATGCTTTCTTTAAGTCAACGGGGTAAGAAGCCCGTGTATCTAACGTTCAGGTAGGAACGCCATCGAGCATGGGGACCTCGAGGTTGTTGCTGTCAACGAGTGAGTGTCCCAAAAGTGTTAATCCATTAGATGAATCACTTAATTGTCCATAGCCCTTTTATTGACTTAGACTACATGGTATGATACTTCTTTCCGCCCAATAGCTTCGTACTGACTCTGTTGCGCAGGTCTACATGTTCAAGTATGACTCCGTAAGTCTCCCAAGTTCTAACGTTCTGTATGGCTTATTAACCTGATTTTCGACAGACACATGGTCGCTTCAAGGGCTCCGTCGAGGTTAAGGACGGTAAACTTTACATCAACAACAAGGCCATTGCCGTCTTCGGCGAGAAAGACCCTGCCAACATCAAGTGGGG |
| Name of Depositor： | KJ Kwon-Chung |
| Isolation： | Single-basidiospore isolate from a cross of ATCC 28957 x ATCC 28958. |
| Cross References： | Nucleotide (GenBank) : AF316889 Filobasidiella neoformans var. neoformans elongation factor 3 mRNA, complete cds. |
| References： | Salas SD, et al. Effect of the laccase gene, CNLAC1, on virulence of cryptococcus neoformans. J. Exp. Med. 184: 377-386, 1996. PubMed: 8760791 Schmeding KA, et al. Sexual compatibility between serotypes of Filobasidiella neoformans (Cryptococcus neoformans). Curr. Microbiol. 5: 133-138, 1981. Kwon-Chung KJ. Morphogenesis of Filobasidiella neoformans, the sexual state of Cryptococcus neoformans. Mycologia 68: 821-833, 1976. PubMed: 790172 Kwon-Chung KJ. Nuclear genotypes of spore chains in Filobasidiella neoformans (Cryptococcus neoformans). Mycologia 72: 418-422, 1980. PubMed: 6991936 Jacobson ES, Compton GM. Discordant regulation of phenoloxidase and capsular polysaccharide in Cryptococcus neoformans. J. Med. Vet. Mycol. 34: 289-291, 1996. PubMed: 8873890 Williamson PR, et al. Melanin biosynthesis in Cryptococcus neoformans. J. Bacteriol. 180: 1570-1572, 1998. PubMed: 9515929 Liu L, et al. Catecholamine oxidative products, but not melanin, are produced by Cryptococcus neoformans during neuropathogenesis in mice. Infect. Immun. 67: 108-112, 1999. PubMed: 9864203 Blakely G, et al. Evolutionary divergence of an elongation factor 3 from Cryptococcus neoformans. J. Bacteriol. 183: 2241-2248, 2001. PubMed: 11244063 Ikeda R, et al. Characterization of a phenol oxidase from Cryptococcus neoformans var. neoformans. Microbiol. Immunol. 37: 759-764, 1993. PubMed: 8289682 Kupfer DM, et al. Introns and splicing elements of five diverse fungi. Eukaryot. Cell 3(5): 1088-1100, 2004. PubMed: 15470237 Meyer W. et al. Consensus multi-locus sequence typing scheme for Cryptococcus neoformans and Cryptococcus gattii. Med. Mycol. 47(6):561-570, 2009. PubMed: 19462334 Hagen F, et al. Recognition of seven species in the Cryptococcus gattii/Cryptococcus neoformans species complex. Fungal Genet. Biol. 78: 16-48, 2015. PubMed: 25721988 Schmeding KA, et al. Sexual compatibility between serotypes of Filobasidiella neoformans (Cryptococcus neoformans). Curr. Microbiol. 5: 133-138, 1981. Kwon-Chung KJ. Morphogenesis of Filobasidiella neoformans, the sexual state of Cryptococcus neoformans. Mycologia 68: 821-833, 1976. PubMed: 790172 Kwon-Chung KJ. Nuclear genotypes of spore chains in Filobasidiella neoformans (Cryptococcus neoformans). Mycologia 72: 418-422, 1980. PubMed: 6991936 |