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微信小章| 产品名称： | Escherichia coli (Migula) Castellani and Chalmers |
|---|---|
| 商品货号： | TS206320 |
| Classification： | Enterobacteriaceae, Escherichia |
| Strain Designations： | LE392 NRRL B-14219 |
| Application： | transformation host |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen |
| Storage Conditions： | Frozen: -80°C or colder Freeze-Dried: 2°C to 8°C Live Culture: See Propagation Section |
| Preceptrol®： | no |
| Genotype： | F- supE44 supF58 lacY1 or deltalac(I-Y)6 galK2 galT22 metB1 trpR55 hsdR514 mcrB lambda- |
| Type Strain： | no |
| Comments： | A host for plating lambda libraries or clones requiring suppression of amber mutations (i.e. Charon 4A, Charon 21A, etc.). Strain LE392 same as NRRL B-14219. |
| Medium： | ATCC® Medium 1065: LB Agar/Broth, Miller |
| Growth Conditions： | Temperature: 37°C
Atmosphere: Aerobic |
| Name of Depositor： | P Leder |
| References： | Murray NE, et al. Lambdoid phages that simplify the recovery of in vitro recombinants. Mol. Gen. Genet. 150: 53-61, 1977. PubMed: 319344 Leder P, et al. EK2 derivatives of bacteriophage lambda useful in the cloning of DNA from higher-organisms: the lambdagtWES system. Science 196: 175-177, 1977. PubMed: 322278 Brunel F, et al. Cloning of bacteriophage T5 DNA fragments in plasmid pBR322 and bacteriophage lambdagtWES. Gene 8: 53-68, 1979. PubMed: 535730 Gowda K, et al. Protein SRP54 of human signal recognition particle: cloning, expression, and comparative analysis of functional sites. Gene 207: 197-207, 1998. PubMed: 9511762 |