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| 产品名称： | Escherichia coli (Migula) Castellani and Chalmers |
|---|---|
| 商品货号： | TS206363 |
| Classification： | Enterobacteriaceae, Escherichia |
| Strain Designations： | WM6 |
| Application： | protease-deficient transformation host transformation host |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | freeze-dried |
| Shipping Information： | Shipped: xa0Freeze dried E. coli |
| Storage Conditions： | Frozen: -80°C or colder Freeze-Dried: 2°C to 8°C Live Culture: See Propagation Section |
| Preceptrol®： | no |
| Genotype： | F- supC(Ts) lac(Am) mal trp(Am) rpsL htpR165 pho(Am) lambda- |
| Type Strain： | no |
| Comments： | The depositor indicated that this strain was derived from CAG-456. The genotype is not complete because the nature of the reversion is not known. This strain has reduced proteolysis of foreign proteins. It does not require heat induction to reduce proteolysis and has the potential to accumulate foreign protein over a longer period of time. |
| Medium： | ATCC® Medium 1065: LB Agar/Broth, Miller |
| Growth Conditions： | Temperature: 30.0°C |
| Name of Depositor： | W Mandecki |
| References： | Mandecki W, et al. High-level expression of a gene encoding the human complement facator C5a in Escherichia coli. Gene 43: 131-138, 1986. PubMed: 3530879 Baker TA, et al. A gene regulating the heat shock response in Escherichia coli also affects proteolysis. Proc. Natl. Acad. Sci. USA 81: 6779-6783, 1984. PubMed: 6387713 |