微信小陈
微信小章
产品简介
购买须知
| 产品名称： | Escherichia coli (Migula) Castellani and Chalmers |
|---|---|
| 商品货号： | TS206364 |
| Classification： | Enterobacteriaceae, Escherichia |
| Strain Designations： | POP2136 DSM 7367 |
| Application： | transformation host |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen |
| Shipping Information： | Shipped: xa0Freeze dried E. coli |
| Storage Conditions： | Frozen: -80°C or colder Freeze-Dried: 2°C to 8°C Live Culture: See Propagation Section |
| Preceptrol®： | no |
| Genotype： | F- supE44 hsdR17 mcrA+ mcrB+ endA1 thi-1 aroB mal- cI857 lambdaPR tetR lambda- |
| Type Strain： | no |
| Comments： | This host is useful for vectors with PR promoters so that expression is regulated by the cI857 repressor. Such vectors include pEX11 (ATCC 37708), pEX12 (ATCC 37709) and pEX13 (ATCC 37710). Constructed by cloning a 2.3 kb BglII fragment containing the cI857 and PR promoter into pOM41 and then transferring into the chromosome of C600. This was introduced into the MM294 background by P1 transduction with aroB. |
| Medium： | ATCC® Medium 1065: LB Agar/Broth, Miller |
| Growth Conditions： | Temperature: 37.0°C |
| Name of Depositor： | BA Van Der Zeijst |
| Chain of Custody： | ATCC < |
| References： | Kusters JG, et al. Improvement of the cloning linker of the bacterial expression vector pEX. Nucleic Acids Res. 17: 8007, 1989. PubMed: 2678010 |