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| 产品名称： | Escherichia coli (Migula) Castellani and Chalmers |
|---|---|
| 商品货号： | TS206630 |
| Classification： | Enterobacteriaceae, Escherichia |
| Strain Designations： | JM103 |
| Application： | transformation host |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen |
| Storage Conditions： | Frozen: -80°C or colder Freeze-Dried: 2°C to 8°C Live Culture: See Propagation Section |
| Preceptrol®： | no |
| Genotype： | F traD36 proA+ proB+ lacIq delta(lacZ)M15 delta(pro-lac) supE hsdR endA1 sbcB15 thi-1 rpsL lambda- |
| Type Strain： | no |
| Comments： | Host strain E. coli JM103. Ten milliter of YT broth is inoculated with a single colony isolate. The overnight culture is diluted 1:10 into YT broth, and incubated for twelve hours and the supernatant is harvested. |
| Medium： | ATCC® Medium 2975: M9 Minimal Agar + 40ug/ml Streptomycin |
| Growth Conditions： | Temperature: 37°C
Atmosphere: Aerobic |
| Name of Depositor： | JC Grosch |
| References： | Hanahan D. Studies on transformation of Escherichia coli with plasmids. J. Mol. Biol. 166: 557-580, 1983. PubMed: 6345791 |