| 产品名称: | Corynebacterium glutamicum (Kinoshita et al.) Abe et al. |
|---|---|
| 商品货号: | TS206779 |
| Strain Designations: | L15 FERM P-5946 |
| Application: | transformation host |
| Biosafety Level: | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format: | freeze-dried |
| Shipping Information: | Distributed: freeze-dried |
| Disclosure: | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Preceptrol®: | no |
| Type Strain: | no |
| Comments: | This is a Corynebacterium glutamicum transformation host. derived from Corynebacterium glutamicum 225-106 (FERM-P5945) Lysozyme-sensitive host for protoplast production and transformation This host is very sensitive to lysozyme (minimum inhibitory concentration = 3.2 ug/ml) facilitating transformation of protoplasts and extraction of DNA. Derived from Corynebacterium glutamicum 225-106 (FERM P-5945). Depositor recommends a medium of 2% powdered bouillon and 0.5% yeast extract. |
| Medium: | ATCC® Medium 3: Nutrient agar or nutrient broth |
| Growth Conditions: | Temperature: 30.0°C |
| Name of Depositor: | Kyowa Ferm. Ind. Co., Ltd., S Wakaki, Kyowa Ferm. Ind. Co., Ltd. |
| U.S. Patent Number: | |
| References: | Katsumata R, et al. Novel lysozyme-sensitive microorganism. US Patent 4,681,847 dated Jul 21 1987 Shigetoshi Wakaki, personal communication |