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微信小章| 产品名称： | Nakazawaea wickerhamii (Capriotti) Kurtzman et C.J. Robnett |
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| 商品货号： | TS207878 |
| Deposited As： | Candida wickerhamii (Capriotti) Meyer et Yarrow, anamorph |
| Strain Designations： | CBS 2928 CCRC 21495, IFO 10322, NRRL Y-2563 |
| Application： | Degrades cellobiose
Degrades cellodextrin
Produces ethyl alcohol ethanol
Produces glucosidase, beta; acid Glucosidase, beta, acid, glucocerebrosidase
Produces ethanol from cellodextrins and cellobiose |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | freeze-dried |
| Storage Conditions： | Frozen: -80°C or colder Freeze-Dried: 2°C to 8°C Live Culture: See Propagation Section |
| Type Strain： | yes |
| Preceptrol®： | no |
| Comments： | Mixed culture (with ATCC 24702) fermentation of cellodextrins to ethanol |
| Morphology： | After 3 days on YM Medium at 25°C, colonies are smooth, white, entire, and butyrous. Cells are globose to subglobose arranged singles and pairs. |
| Medium： | ATCC® Medium 28: Emmons modification of Sabourauds agar ATCC® Medium 200: YM agar or YM broth ATCC® Medium 1245: YEPD |
| Growth Conditions： | Temperature: 24°C to 26°C
Atmosphere: Typical aerobic |
| Sequenced Data： |
18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence AAGGATCATTACAGTATTCTATTGCCAGCGCTTAACTGCGCGGCGATAAACCTTACACACTTTGTTTTTATTATTGAAGACACCATTGCTTTGGTGGGTAAGTGTAAAAGCTTTATCCGCCAGAGGTTTTATTAAAACTCTATATATTAAAGGTAACTTTTTTAAATAGTCGATATATTACATTTGCTGATTAATTTCGGTGGATACAAAATATTTAAAACTTTCAACAACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAATTGCGATAAGTATTGTGAATTGCAGAATTTCGTGAATCATCGAATCTTTGAACGCACATTGCACCCTGTGGTATTCCACAGGGTATGCCTGTTTGAGCGTCATTTCTCTCTCAAAACACTAGTTTTGGTATTGAGTGATACTCGGGTTATAGGCTTGAGTTTGCTTGAAAAGTATTGGCAAGGGAGACAGTTCTCGAGAAAGCTATTTATTCAATGTATTAGGTTTATCCAACTCGTTGATTGATGGTGGGATTGAGTGATTGTTATACAGGCCCGGCCTTACAACAAACAAACCAAAGTTTGACCTCAAATCAGGTAGGATTACCCGCTGAACTTAAG D1D2 region of the 26S ribosomal RNA gene CATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCCTTAGTAACGGCGAGTGAAGCGGCAAGAGCTCAAATTTGAAATCTAGCACTTTCAGTGTTCGAGTTGTAATTTGAAGAAGGTATCTTTGGAGTTGGCCCCTGTCTATGTTCCTTGGAACAGGACGTCACAGAGGGTGAGAATCCCGTGCGATGGGGAGACCAATTCTTTGTAAAGTTCCTTCGAAGAGTCGAGTTGTTTGGGAATGCAGCTCTAAGTGGGTGGTAAATTCCATCTAAAGCTAAATATTGGCGAGAGACCGATAGCGAACAAGTACAGTGATGGAAAGATGAAAAGAACTTTGAAAAGAGAGTGAAAAAGTACGTGAAATTGTTGAAAGGGAAGGGCTTTTGATCAGACTTGGTGTTTAGTACTCTCTACTCCTTGTGGGTGGGGCTCTTGCTTTATACTGGGCCAGCATCGGTTTGGATGGTAGGATAATGGCATTGGAACGTGGCTCCACTTGTGGAGTGTTATAGCCCTTGCTGATGCTACCTATCTAGACCGAGGACTGCGTCTTTGACTAGGATGCTGGCGTAATGATTAAAAGCCGC |
| Name of Depositor： | CBS |
| Chain of Custody： | ATCC <-- CBS <-- A. Capriotti |
| Isolation： | Silage mixture containing olive husks, molasses, and whey |
| References： | Gonde P, et al. Fermentation of cellodextrins by different yeast strains. Appl. Environ. Microbiol. 48: 265-269, 1984. Freer SN, Wing RE. Fermentation of cellodextrins to ethanol using mixed-culture fermentations. Biotechnol. Bioeng. 27: 1085-1088, 1985. Capriotti A. Torulopsis wickerhamii nova species. A new yeast from silage. Arch. Mikrobiol. 30: 383-386, 1958. PubMed: 13595817 Skory CD, Freer SN. Cloning and characterization of a gene encoding a cell-bound, extracellular beta-glucosidase in the yeast Candida wickerhamii. Appl. Environ. Microbiol. 61: 518-525, 1995. PubMed: 7574590 Freer SN. Kinetic characterization of a beta-glucosidase from a yeast, Candida wickerhamii. J. Biol. Chem. 268: 9337-9342, 1993. PubMed: 8486628 Freer SN, Detroy RW. Direct fermentation of cellodextrins to ethanol by Candida wickerhamii and C. lusitaniae. Biotechnol. Lett. 4: 453-458, 1982. Leclerc M, et al. The enzyme systems in a strain of Candida wickerhamii Meyer and Yarrow participating in the hydrolysis of cellodextrins. J. Gen. Appl. Microbiol. 30: 509-521, 1984. Freer SN, Detroy RW. Characterization of cellobiose fermentations to ethanol by yeasts. Biotechnol. Bioeng. 25: 541-557, 1983. Kurtzman CP, Robnett CJ. Description of Kuraishia piskuri f.a., sp. nov., a new methanol assimilating yeast and transfer of phylogenetically related Candida species to the genera Kuraishia and Nakazawaea as new combinations. FEMS Yeast Res. 14: 1028-1036, 2014. PubMed: 25132542 |