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微信小章| 产品名称： | SU-DHL-4 |
|---|---|
| 商品货号： | TS209402 |
| Organism： | Homo sapiens, human |
| Tissue： | peritoneal effusion |
| Cell Type： | B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast-like |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | 38 years |
| Gender： | male |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Images： |  |
| Clinical Data： | 38 years Caucasian male |
| Genes Expressed： | IgG+, Kappa+, IgM-, IgA-, IgD-, Lambda-.xa0 This cell line has relatively high expression levels of Bax, Bak, AIF, and high caspase-9 activity. |
| Comments： | The depositor states that this cell line is negative for Epstein-Barr Virus (EBV). This cell line has a 14;18 chromosomal translocation. This cell line has a major rearrangement in the BCL-2 gene. This cell line is negative for Candida ingestion. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
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| Subculturing： | Cultures can be maintained by the addition of fresh medium. An inoculum of 1.0 x 105to 2.0 x 105cells/mL is recommended. Subculture when the cell concentration is between 8.0 x 105 and 1.0 x 106 cells/mL. Subcultivation ratio: A subcultivation ratio of 1:2 to 1:6 is recommended Medium renewal: every 2 to 4 days (depending on cell density) |
| Cryopreservation： | Storage temperature:xa0liquid nitrogen vapor phase Freeze medium:xa0complete growth medium supplemented with an additional 20% fetal bovine serum and 10% (v/v) DMSO |
| Culture Conditions： | Temperature: 37°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| STR Profile： | D5S818: 11, 12 D13S317: 11, 12 D7S820: 8, 11 D16S539: 11, 13 vWA: 18, 19 THO1: 6, 9.3 Amelogenin: X Y TPOX: 9, 11 CSF1PO: 12 |
| Name of Depositor： | A Epstein |
| Year of Origin： | 1976 |
| References： | Kaiser-McCaw Hecht B, et al. Histiocytic lymphoma cell lines: immunologic and cytogenetic studies. Cancer Genet. Cytogenet. 14 (3-4): 205-218, 1985. PubMed: 3881165 Siminovitch KA, et al. Immunoglobulin gene rearrangements and expression in diffuse histiocytic lymphomas reveal cellular lineage, molecular defects, and sites of chromosomal translocation. Blood. 67(2): 391-397, 1986. PubMed: 3080039 Epstein AL, Kaplan HS. Feeder layer and nutritional requirements for the establishment and cloning of human malignant lymphoma cell lines. Cancer Res. 39(5):1748-1759, 1979. PubMed: 371794 Bakhshi A, et al. Cloning the chromosomal breakpoint of t(14;18) human lymphomas: clustering around JH on chromosome 14 and near a transcriptional unit on 18. Cell. 41(3):899-906, 1985. PubMed: 3924412 |