微信小陈
微信小章| 产品名称： | P3/NSI/1-Ag4-1 NS-1 |
|---|---|
| 商品货号： | TS209409 |
| Organism： | Mus musculus, mouse |
| Cell Type： | B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | plasmacytoma; myeloma |
| Strain： | BALB/c |
| Applications： | Kappa chains are synthesized but not secreted. Tested and found negative for ectromelia virus (mousepox). The cells are resistant to 0.1 mM 8-azaguanine and do not grow in HAT medium. |
| Storage Conditions： | liqid nitrogen vapor phase |
| Images： |  |
| Derivation： | This is a non-secreting clone of P3X63Ag8 (ATCC TIB-9). |
| Antigen Expression： | Antigen expression: H-2d |
| Genes Expressed： | immunoglobulin (not secreted),H-2d |
| Cellular Products： | immunoglobulin (not secreted) |
| Comments： | Kappa chains are synthesized but not secreted. The cells are resistant to 0.1 mM 8-azaguanine and do not grow in HAT medium. The cells have been reported to be cholesterol auxotrophs due to a deficiency in 3-ketosteroid reductase activity. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 105 viable cells/mL. Maintain cell density between 1 X 105 and 1 X 106 viable cells/mL. Do not allow the cell density to exceed 1 X 106 cells/mL.
Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liqid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| Isotype： | kappa light chain |
| Name of Depositor： | G Kohler, C Milstein |
| Deposited As： | Mus musculus |
| References： | Barnstable CJ, et al. Production of monoclonal antibodies to group A erythrocytes, HLA and other human cell surface antigens -- new tools for genetic analysis. Cell 14: 9-20, 1978. PubMed: 667938 Kohler G, et al. Fusion between immunoglobulin-secreting and nonsecreting myeloma cell lines. Eur. J. Immunol. 6: 292-295, 1976. PubMed: 825374 Horibata K, Harris AW. Mouse myelomas and lymphomas in culture. Exp. Cell Res. 60: 61-77, 1970. PubMed: 5439579 Kohler G, Milstein C. Derivation of specific antibody-producing tissue culture and tumor lines by cell fusion. Eur. J. Immunol. 6: 511-519, 1976. PubMed: 825377 Sato JD, et al. Effects of proximate cholesterol precursors and steroid hormones on mouse myeloma growth in serum-free medium. In Vitro Cell. Dev. Biol. 24: 1223-1228, 1988. PubMed: 3209588 Ramasamy R, et al. Possible role for the Fc receptor on B lymphocytes. Nature 249: 573-574, 1974. PubMed: 4545851 Cowan NJ, et al. Intracellular immunoglobulin chain synthesis in non-secreting variants of a mouse myeloma: detection of inactive light-chain messenger RNA. J. Mol. Biol. 90: 691-701, 1974. PubMed: 4449137 Condie R, et al. Cannabinoid inhibition of adenylate cyclase-mediated signal transduction and interleukin 2 (IL-2) expression in the murine T-cell line, EL4.IL-2. J. Biol. Chem. 271: 13175-13183, 1996. PubMed: 8662742 |
| Cross References： | Nucleotide (GenBank) : AF248032 Mus musculus coatomer protein alpha subunit precursor, mRNA, partial cds. |