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微信小章| 产品名称： | CE3 |
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| 商品货号： | TS209415 |
| Organism： | Mus musculus, mouse |
| Tissue： | inner cell mass |
| Cell Type： | embryonic stem cell |
| Product Format： | frozen |
| Morphology： | spherical colony |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | embryo |
| Strain： | 129S2/SvPas |
| Applications： | Neural differentiated CE3 cells are intensely GFP+ and suitable for cell tracking in tissue culture and transplantation.
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| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | The CE3 cell line was derived from the D3 ES cell line PubMed: 12591158.
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| Comments： | CE3 (for Cassette Exchange) contains one acceptor module that allows for efficient double lox targeting, with constitutive GFP expression. Neural differentiated CE3 cells are intensely GFP+ and suitable for cell tracking in tissue culture and transplantation. The cell line is also puromycin resistant. The CE3 cell line was derived from the D3 ES cell line PubMed: 12591158. |
| Complete Growth Medium： | Grow ES cells in Mouse ES Cell Basal Medium (ATCC SCRR-2011) that has been supplemented with the following components: 1. 0.1 mM 2-mercaptoethanol (Life Technologies Cat. No. 21985-023) 2. 1,000 U/mL mouse leukemia inhibitory factor (LIF) (Millipore Cat. No. ESG1107) 3. 10% to 15% ES-Cell Qualified FBS (ATCC® SCRR-30-2020) or an ES cell qualified serum replacement Complete Growth Medium for Mouse ES Cells is stable for 14 days when stored at 2°C to 8°C. |
| Subculturing： | To insure the highest level of viability, pre-warm media and Trypsin/EDTA to 37ºC before adding to cells. Volumes used in this protocol are for T75 flasks. Proportionally adjust the volumes for culture vessels of other sizes. A split ratio of 1:4 to 1:7 is recommended. Feeder Cell Preparation for Subcultures
Dissociation and Transfer of ES Cells
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| Cryopreservation： | Freeze medium: Complete growth medium supplemented with an additional 10% FBS and 10% DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| Name of Depositor： | DI Gottlieb |
| References： | Matise M, et alProduction of targeted embryonic stem cell clonesIn: Matise M, et alGene Targeting: A Practical ApproachOxfordOxford University Press101-132, 1999 Adams LD, et al. Double lox targeting for neural cell transgenesis. Brain Res. Mol. Brain Res. 110: 220-233, 2003. PubMed: 12591158 |