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微信小章| 产品名称： | Mino |
|---|---|
| 商品货号： | TS209416 |
| Organism： | Homo sapiens, human |
| Tissue： | peripheral blood/Mantle cell lymphoma (B cell non-Hodgkins lymphoma) |
| Cell Type： | lymphoblast; cytogenetic abnormalit
Ref  Lai R, et al. Establishment and characterization of a new mantle cell lymphoma cell line, Mino. Leuk. Res. 26: 849-855, 2002. PubMed: 12127561 |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Mantle Cell Lymphoma |
| Age： | 64 |
| Gender： | male |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | Modal chromosome number: 74. This is a hypertriploid male cell line, del(6)(q16), t(8;11)(q22;q23), add(11)(q25), add(13)(q34), add(14)(q32). Most cells contain the t(11;14) derivative chromosome, detected by FISH with whole chromosome paint probes. |
| Images： |  |
| Clinical Data： | 64 Caucasian male |
| Antigen Expression： | CD3-, CD5+, CD10-, CD19+, CD20+, CD23- (verified at ATCC) |
| Oncogene： | p53, p16 (INK4a), and p21(WAF1) |
| Genes Expressed： | cyclin D2 and cyclin D3, p53, p16 (INK4a), and p21(WAF1) |
| Comments： | The cells are large, growing singly and in small clumps in vitro. The immunophenotype by flow cytometry is compatible with MCL.
Western blots show expression of cyclin D1 but no detectable cyclin D2 and cyclin D3; the retinoblastoma protein is predominantly phosphorylated. There is expression of tumor suppressor gene products including p53, p16 (INK4a), and p21(WAF1). Ref Lai R, et al. Establishment and characterization of a new mantle cell lymphoma cell line, Mino. Leuk. Res. 26: 849-855, 2002. PubMed: 12127561 |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 15%.
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| Subculturing： | Cultures can be maintained by the addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 6 X 104 viable cells/mL. Do not allow the cell concentration to reach 1.5 X 106 cells/mL. Subcultivation Ratio: 1:5 to 1:10 |
| Cryopreservation： | 90% FBS; 10% (v/v) DMSO |
| Culture Conditions： | Temperature: 37.0°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| STR Profile： | Amelogenin: X,Y
CSF1PO: 9,11 D13S317: 12 D16S539: 11,12 D5S818: 11,12 D7S820: 10,11 THO1: 9.3 TPOX: 8,11 vWA: 14,17 |
| Population Doubling Time： | about 72 hours |
| Name of Depositor： | R.J.Ford |
| References： | Lai R, et al. Establishment and characterization of a new mantle cell lymphoma cell line, Mino. Leuk. Res. 26: 849-855, 2002. PubMed: 12127561 Marzec M, et al. Mantle cell lymphoma cells express predominantly cyclin D1a isoform and are highly sensitive to selective inhibition of CDK4 kinase activity. Blood 108: 1744-1750, 2006. PubMed: 16690963 Salaverria I, et al. Mantle cell lymphoma: from pathology and molecular pathogenesis to new therapeutic perspectives. Haematologica 91: 11-16, 2006. PubMed: 16434365 Lai R, et al. Establishment and characterization of a new mantle cell lymphoma cell line, Mino. Leuk. Res. 26: 849-855, 2002. PubMed: 12127561 |