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微信小章| 产品名称： | VMM5A |
|---|---|
| 商品货号： | TS209417 |
| Organism： | Homo sapiens, human |
| Tissue： | Melanoma, Lymph Node Metastasis |
| Cell Type： | Melanocyte |
| Product Format： | frozen |
| Morphology： | Epithelial-like |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Melanoma, Stage IIIC; malignant |
| Age： | 81 |
| Gender： | Male |
| Ethnicity： | Caucasian |
| Applications： | Drug screening Development of targeted therapy Development of combination therapy Tumor vaccine development |
| Storage Conditions： | liquid nitrogen vapor phase |
| Images： |  |
| Derivation： | Derived from tumors taken from tumor-involved lymph nodes from patients at the University of Virginia |
| Clinical Data： | Primary Site: Right Posterior Shoulder; Metastatic Site: Lymph Node, Right Neck |
| Antigen Expression： | Positives: High VEGF-R2, Low GP100, Tyrosinase, Low MAGE-A1, MMP-1 |
| HLA Typing： | A2.1,A1101,B39,B44, BW4,BW6,C7(17)DR7, DR8,DR11,DQ2,DQ7 |
| Comments： | NRAS: wt CDKN2A: wt BRAF Mutation: V600E APC Mutation PDGFRA Mutation: D842V PTEN Mutation: R173Hxa0 more sensitive to BAY43-9006 (BRAF kinase inhibitor) and to rapamycin (mTOR kinase inhibitor), compared to cell lines with wild-type B-Raf |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
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| Subculturing： | Volumes are for a T-75 flask; Adjust accordingly
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| Cryopreservation： | Fetal bovine serum, 90%; DMSO, 10% |
| Culture Conditions： | Temperature: 37°C Atmosphere: air, 95%; carbon dioxide (CO2 ), 5% |
| STR Profile： | Amelogenin: X,Y D5S818: 9 D7S820: 9,10 D13S317: 12 D16S539: 12 vWA: 16 TH01: 9.3 TPOX: 8,11 CSF1PO: 11 |
| Sterility Tests： | Pass |
| Name of Depositor： | Craig L. Slingluff, Jr. M.D. |
| Passage History： | Unknown. 2 passages from 2003 frozen cell line stock, but it is not known how long the line was in culture after being established from tumor tissue obtained in 1992. |
| Year of Origin： | 1992 |
| References： | Slingluff C, et al. Melanomas with concordant loss of multiple melanocytic differentiation proteins: immune escape that may be overcome by targeting unique or undefined antigens. Cancer Immunol. Immunother. 48:661-672, 2000. PubMed: 10752474 Molhoek K, et al. Human melanoma cytolysis by combined inhibition of mammalian target of rapamycin and Vascular Endothelial Growth Factor/Vascular Endothelial Growth Factor Receptor-2. Cancer Res. 68: (11), 2008. PubMed: 18519701 Molhoek K, et al. Comprehensive analysis of RTK activation in human melanomas reveals autocrine signaling through IGF-1R. Melanoma Res 21(4): 274–284, 2011. PubMed: 21654344 Molhoek K, et al. Synergistic inhibition of human melanoma proliferation by combination treatment with B-Raf inhibitor BAY43-9006 and mTOR inhibitor Rapamycin. J. Trans. Med. 3(39): 2005. PubMed:16255777 |