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| 产品名称： | HMEC-1 |
|---|---|
| 商品货号： | TS209418 |
| Organism： | Homo sapiens, human |
| Tissue： | dermal endothelium |
| Cell Type： | dermal microvascular endothelium |
| Product Format： | frozen |
| Morphology： | endothelial-like |
| Culture Properties： | adherent |
| Biosafety Level： | 2 xa0Cells contain SV-40 viral DNA sequences
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | newborn |
| Gender： | male |
| Applications： | angiogenesis, leukocyte trafficking, wound healing, inflammation, circulation, tumor growth and metastasis.cancer research, endothelium function, cell trafficking, surface molecule interactions, drug screening, toxicological studies for the pharmaceutical and cosmetic industry |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | Microvascular endothelial cells isolated from human foreskins were transfected with pSVT vector, xa0a pBR-322 based plasmid containing the coding region for Simian virus 40A gene product, large T antigen. |
| Receptor Expression： | The cells express von Willebrands factor (vWF), cell adhesion molecules ICAM-1 and are capable of acetylated LDL uptake. Ref (Ades EW, et al. HMEC-1: Establishment of an Immortalized Human Microvascular Endotheilal Cell Line. J. Invest. Dermatol. 99(6): 683-690, 1992. PubMed: 1361507) |
| Comments： | CRL-3243, HMEC-1, has been well characterized and shown to retain many of the characteristics of endothelial cells. These immortalized cells, because they are a continously renewable source of human endothelial microvascular cells, can be used as a replacement for primary human dermal endothelial cells for many research studies. |
| Complete Growth Medium： | The base medium for this cell line is MCDB131 (without L-Glutamine). To make the complete growth medium, add the following components to the base medium:
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.xa0
Subcultivation Ratio: 1:6 to 1:12 is recommended. Medium Renewal: Do not feed cells with growth medium between subcultures or cells become very tightly attached and will be difficult to disperse with trypsin-EDTA |
| Cryopreservation： | Freeze Medium: Complete growth medium, 92.5%; DMSO, 7.5%
Storage Temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C |
| STR Profile： | Amelogenin: X,Y CSF1PO: 10, 12 D13S317: 11, 12 D16S539: xa011, 12 D5S818: 12 D7S820: 8, 10 THO1: 7 TPOX: 8, 11 vWA: 15, 17 |
| Name of Depositor： | Kathryn Kellar, Ph. D. |
| Year of Origin： | November 1990 |
| References： | Ades EW, et al. HMEC-1: Establishment of an Immortalized Human Microvascular Endotheilal Cell Line. J. Invest. Dermatol. 99(6): 683-690, 1992. PubMed: 1361507 |