微信小陈
微信小章
产品简介
购买须知
| 产品名称： | SU-DHL-10 |
|---|---|
| 商品货号： | TS209421 |
| Organism： | Homo sapiens, human |
| Tissue： | lymph node Derived from Metastatic Site: peritoneal effusion |
| Cell Type： | B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast-like |
| Culture Properties： | suspension, multicellular aggregates |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Large Cell Lymphoma |
| Age： | 25 years |
| Gender： | Male |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Images： |  |
| Clinical Data： | 25 years Caucasian Male |
| Comments： | ATCC confirmed this cell line is negative for the presence of Epstein-Barr viral DNA sequences via PCR. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing： | Cultures can be maintained by the addition of fresh medium. An inoculum of 8.0 x 104 to 2.0 x 105cells/mL is recommended. Subculture when the cell concentration is between 1.0 x 106 and 2.0 x 106 cells/mL. Subcultivation ratio: A subcultivation ratio of 1:4 to 1:12 is recommended. Medium renewal: every 2 to 4 days |
| Cryopreservation： | Freeze medium: complete growth medium supplemented with an additional 20% fetal bovine serum and 10% DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37.0°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| STR Profile： | D5S818: 11, 12 |
| Isotype： | IgG lambda |
| Name of Depositor： | A Epstein |
| Year of Origin： | 1978 |