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| 产品名称： | STC-1 |
|---|---|
| 商品货号： | TS209424 |
| Organism： | Mus musculus, mouse |
| Tissue： | intestine |
| Cell Type： | intestinal neuroendocrine tumor cells |
| Product Format： | frozen 1.0 mL |
| Morphology： | epithelial-like |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | invasive small intestinal neuroendocrine carcinoma |
| Age： | 10 to 13 weeks |
| Strain： | C57B1/6J |
| Applications： | This cell line may be a useful model for human neuroendocrine neoplasms of the gut, and useful tools for studying hormone secretin. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Images： |  |
| Derivation： | The STC-1 cell line was derived from the intestinal tumors of RIP1Tag2/Rip2pyST1xa0double transgenic mice. |
| Cellular Products： | secretin |
| Comments： | The STC-1 cell line was derived from the intestinal tumors of double transgenic mice. Transgenic mice harboring a hybrid gene linking the rat insulin promoter (RIP) to polyoma small T (PyST) antigen were mated with transgenic mice harboring rat insulin promoter (RIP) linked to SV40 early region (Tag) creating off-spring harboring both transgenes (double transgenics). These mice were found to have frequent intestinal tumors in addition to pancreatic Beta-cell tumors. Gene expression studies suggested that the intestinal and pancreatic tumors arose as separate entities. The STC-1cell line produces the hormone secretin. This cell line may be a useful model for human endocrine neoplasms of the gut. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Cells must be subcultured when they reach ~70% confluence, or else they start to come off the flask into suspension. Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.xa0
Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 10% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°Cxa0 Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| Cells per Vial： | 1.0 x 10^6 |
| Volume： | 1.0 mL |
| Name of Depositor： | Douglas Hanahan, PhD Cold Spring Harbor Laboratory |
| Year of Origin： | June 1990 |
| References： | Rindi G, et al. Development of Neuroendocrine Tumors in the Gastrointestinal Tract of Transgenic Mice. Am J Pathol 136(6): 1349-1363, 1990. PubMed: 2162628 Grant SGN, et al. Early Invasiveness Characterizes Metastatic Carcinoid Tumors in Transgenic Mice. Cancer Res 51: 4917-4923, 1991. PubMed: 1654206 |