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微信小章| 产品名称： | SU-DHL-2 |
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| 商品货号： | TS209428 |
| Organism： | Homo sapiens, human |
| Tissue： | lymph node Derived from Metastatic Site: peritoneal effusion |
| Cell Type： | histiocytic |
| Product Format： | frozen |
| Morphology： | Lymphoblast-like |
| Culture Properties： | suspension, multicell aggregates |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | large cell lymphoma; diffuse histiocytic lymphoma |
| Age： | 73 years |
| Gender： | Female |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | Most of these cells were hyperdiploid, with a sharp modal number of 51 chromosomes; occasional cells in the hypertetraploid range were also seen. A minute marker chromosome was consistently observed, and increased numbers of chromosomes were seen in the A, B, C, and F groups. |
| Images： |  |
| Clinical Data： | 73 years Caucasian Female |
| Comments： | The cells are non-specific esterase and acid phosphatase positive. The cells are negative for Epstein-Barr virus nuclear antigen (EBNA-). The cells are surface Ig negative (sIG-). The cells are reported to be E - rosette negative. ATCC confirmed this cell line is positive for the presence of Epstein-Barr viral DNA sequences via PCR. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
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| Subculturing： | Cultures can be maintained by the addition of fresh medium. An inoculum of 1 X 105 to 3 x 105 cells/mL is recommended. Subculture when cell concentration is between 7 x 105 and 1 x 106 cells/mL. Subcultivation ratio: A subcultivation ratio of 1:3 to 1:8 is recommended. Medium renewal: Add fresh medium every 2 to 3 days (depending on cell density) |
| Cryopreservation： | Storage temperature: liquid nitrogen vapor phase Freeze medium: complete growth medium supplemented with an additional 20% fetal bovine serum and 10% (v/v) DMSO |
| Culture Conditions： | Temperature: 37.0°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| STR Profile： | D5S818: 13 D13S317: 11, 12 D7S820: 11, 13 D16S539: 14 vWA: 17 THO1: 6, 9 TPOX: 8 CSF1PO: 12 Amelogenin: X |
| Name of Depositor： | A Epstein |
| Year of Origin： | 1974 |
| References： | Epstein AL, et al. Biology of the human malignant lymphomas. IV. Functional characterization of ten diffuse histiocytic lymphoma cell lines. Cancer. 42(5): 2379-2391, 1978. PubMed: 214220 Siminovitch KA, et al. Immunoglobulin gene rearrangements and expression in diffuse histiocytic lymphomas reveal cellular lineage, molecular defects, and sites of chromosomal translocation. Blood. 67(2): 391-397, 1986. PubMed: 3080039 Epstein AL, Kaplan HS. Feeder layer and nutritional requirements for the establishment and cloning of human malignant lymphoma cell lines. Cancer Res. 39(5):1748-1759, 1979. PubMed: 371794 |