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微信小章| 产品名称： | PNEC30 |
|---|---|
| 商品货号： | TS209430 |
| Organism： | Mus musculus, transgenic, mouse, transgenic |
| Tissue： | prostate |
| Cell Type： | neuroendocrine |
| Product Format： | frozen |
| Morphology： | neuronal |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | prostate neuroendocrine cancer |
| Age： | 6 months |
| Gender： | male |
| Applications： | PNEC cell lines should be useful for genetic and/or pharmacologic studies of the regulation of NE cell proliferation, differentiation, and tumorigenesis. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Images： |  |
| Derivation： | Prostate neuroendocrine cancer (PNEC) cell lines were established from CR2-TAg prostate tumors and metastases. |
| Clinical Data： | 6 months
male |
| Receptor Expression： | Gamma-aminobutyric acid (GABAA), expressed Ref   Ippolito JE, et al. An integrated functional genomics and metabolomics approach for defining poor prognosis in human neuroendocrine cancers. Proc. Natl. Acad. Sci. 102(28):9901-9906, 2005. PubMed: 15998737 |
| Genes Expressed： | mAsh1 transcription factor, expressed |
| Cellular Products： | mAsh1 transcription factor, expressed |
| Tumorigenic： | YES
RefHu Y, et al. RNA interference of achaete-scute homolog 1 in mouse prostate neuroendocrine cells reveals its gene targets and DNA binding sites. Proc. Natl. Acad. Sci. 101(15):5559-5564, 2004. PubMed: 15060276 |
| Effects： | tumorigenic in BALB/c mice |
| Comments： | GeneChip analyses of cell lines harvested at different passages, and as xenografted tumors, indicated that PNECs express consistent features ex vivo and in vivo and share a remarkable degree of similarity with primary CR2-TAg prostate neuroendocrine (NE) tumors.xa0 PNECs express mAsh1 (mouse homolog proneural gene complex achaete-scute), a basic helix-loop-helix (bHLH) transcription factor essential for NE cell differentiation in other tissues. PNEC cell lines should be useful for genetic and/or pharmacologic studies of the regulation of NE cell proliferation, differentiation, and tumorigenesis. PNEC30 cells, when cultured on non-coated surfaces, grow in suspension as multicellular aggregates that resemble the neurospheres of cultured neural stem cells. |
| Complete Growth Medium： | The base medium for this cell line is Neural Progenitor Basal Medium, which is supplied as part of the NPMM Neural Progenitor Maintenance Medium Bullet Kit available from Lonza/Clonetics Inc., Catalog No. CC-3209. To make the complete growth medium, add the following components to 500 ml of the base medium:
Note: Do not filter complete medium. |
| Subculturing： | Volumes used in this protocol are for 75 cm2. flasks; proportionally reduce or increase amount of solutions for culture vessels of other sizes. Note: These cells are cultured on poly-D-lysine coated vessels (BD BioCoat Cellware, BD Biosciences, Cat. No. 356524 for 75 cm2. flask) which are additionally coated with 20 μg/mL laminin (Sigma, Cat. No. L2020 or equivalent). Add 5 mL laminin solution to a 75 cm2. flask and incubate overnight at room temperature. Remove laminin solution and allow flask to air dry uncapped and standing upright in a biological cabinet before introducing cells.xa0
Subcultivation ratio: A subcultivation ratio of 1:3 to 1:8 is recommended. Medium renewal: Every 2 to 3 days.
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| Cryopreservation： | Freeze medium: fetal bovine serum, 90%; DMSO, 10% liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| Population Doubling Time： | approximately 50 hours |
| Name of Depositor： | J Gordon and J Ippolito |
| Year of Origin： | 2002 |
| References： | Ippolito JE, et al. An integrated functional genomics and metabolomics approach for defining poor prognosis in human neuroendocrine cancers. Proc. Natl. Acad. Sci. 102(28):9901-9906, 2005. PubMed: 15998737 Hu Y, et al. RNA interference of achaete-scute homolog 1 in mouse prostate neuroendocrine cells reveals its gene targets and DNA binding sites. Proc. Natl. Acad. Sci. 101(15):5559-5564, 2004. PubMed: 15060276 Hu Y, et al. RNA interference of achaete-scute homolog 1 in mouse prostate neuroendocrine cells reveals its gene targets and DNA binding sites. Proc. Natl. Acad. Sci. 101(15):5559-5564, 2004. PubMed: 15060276 |