微信小陈
微信小章| 产品名称： | K7M2 wt K7M2-WT |
|---|---|
| 商品货号： | TS209444 |
| Organism： | Mus musculus, mouse |
| Tissue： | bone; derived from metastatic site: lung |
| Cell Type： | osteoblast |
| Product Format： | frozen |
| Morphology： | osteoblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | osteosarcoma |
| Strain： | BALB/c |
| Applications： | This cell line is a suitable tumor model. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Antigen Expression： | CD31 + Ref |
| Genes Expressed： | secreted phosphoprotein 1 (osteopontin)xa0
factor VIIIxa0Ref integrin binding sialoprotein (BSP)xa0Ref biglyanxa0Ref villin 2 (ezrin)xa0Ref |
| Cellular Products： | secreted phosphoprotein 1 (osteopontin)xa0
factor VIII Ref xa0integrin binding sialoprotein (BSP) Ref xa0biglyan Ref xa0villin 2 (ezrin) Ref |
| Tumorigenic： | Yes |
| Effects： | Yes, forms tumors in BALB/c mice with spontaneous metastasis to the lungs in over 90% of mice inoculated |
| Comments： | Expression of bone sialoprotein, biglyan, decorrin, and osteopontin is suggestive of bone lineage cells. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: 1:4 to 1:6 Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture of Animal Cells: a Manual of Basic Technique by R. Ian Freshney, 5th edition, published by Wiley-Liss, N.Y., 2005 |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
| Population Doubling Time： | 31 hours |
| Name of Depositor： | C Khanna |
| Year of Origin： | 1998 |
| References： | Khanna C, et al. An orthotopic model of murine osteosarcoma with clonally related variants differing in pulmonary metastatic potential. Clin. Exp. Metastasis 18: 261-271, 2000. PubMed: 11315100 Khanna C, et al. Metastasis-associated differences in gene expression in a murine model of osteosarcoma. Cancer Res. 61: 3750-3759, 2001. PubMed: 11325848 Khanna C, et al. The membrane-cytoskeleton linker ezrin is necessary for osteosarcoma metastasis. Nat. Med. 10: 182-186, 2004. PubMed: 14704791 The K7M2 wt cell line was derived by intra-osseous injection of the K7 murine osteosarcoma cell line to the proximal tibia of a BALB/c mouse. The resultant primary tumor spontaneously metastasized to the lungs. A pulmonary metastasis was removed from the lung and then surgically implanted to a paraosteal tibial muscle flap of a naïve mouse. This cycle of implantation of pulmonary metastasis to paraosteal tibial muscle flap sites was repeated, resulting in the K7M2 primary tumor. This tumor was dissociated to a single cell suspension to create the K7M2 wt cell line |
Khanna C, et al. An orthotopic model of murine osteosarcoma with clonally related variants differing in pulmonary metastatic potential. Clin. Exp. Metastasis 18: 261-271, 2000. PubMed: 11315100