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微信小章| 产品名称： | MM.1S |
|---|---|
| 商品货号： | TS209445 |
| Organism： | Homo sapiens, human |
| Tissue： | peripheral blood |
| Cell Type： | B lymphoblast |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | mixed, suspension and lightly attached cells |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | immunoglobulin A lambda myeloma |
| Age： | 42 years
Ref  Greenstein S, et al. Characterization of the MM.1 human multiple myeloma (MM) cell lines: A model system to elucidate the characteristics, behavior, and signaling of steroid-sensitive and -resistant MM cells. Exp. Hematol.: 31:271-282, 2003. PubMed; 12691914 |
| Gender： | female |
| Ethnicity： | Black |
| Applications： | Together, CRL-2974 and CRL-2975 provide critical information about disease progression and the development of drug resistance. These cell lines represent valuable tools for elucidating the mechanisms of action of glucocorticoids and the development of new therapeutics. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Images： |  |
| Derivation： | The parent cell line, MM.1, was established from peripheral blood of a multiple myeloma patient who had become resistant to steroid-based therapy. The closely related cell line, MM.1R (ATCC CRL-2975) was also isolated from MM.1, but is resistant to dexamethasone. |
| Clinical Data： | Black
female
42 years |
| Antigen Expression： | CD25+, CD38+, CD52+, CD59+ Ref Greenstein S, et al. Characterization of the MM.1 human multiple myeloma (MM) cell lines: A model system to elucidate the characteristics, behavior, and signaling of steroid-sensitive and -resistant MM cells. Exp. Hematol.: 31:271-282, 2003. PubMed; 12691914 |
| Receptor Expression： | glucocorticoid receptor, expressed |
| Genes Expressed： | lambda-light chain immunoglobulin |
| Cellular Products： | lambda-light chain immunoglobulin |
| Comments： | MM.1S is sensitive to dexamethasone.xa0
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| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
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| Subculturing： | This is a mixed cell culture; cells grow both as a lightly attached monolayer and in suspension. Cultures can be maintained by adding fresh medium. Alternatively, subcultures can be prepared by scraping the adherent cells into the medium containing the floating cells, collecting the cells by centrifugation, resuspending the cell pellet in fresh medium and dispensing into new flasks.xa0Corning® T-75 flasks (catalog #431464) are recommended for subculturing this product. Note: Subculture cells before they reach confluence; cells in over-confluent cultures begin to form rosettes with necrotic centers.
Subcultivation ratio: A subcultivation ratio of 1:2 to 1:4 is recommended. Medium renewal: Every 2 to 3 days |
| Cryopreservation： | Freeze medium: complete growth medium, 90%; DMSO, 10% liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| STR Profile： | D5S818: 8, 12 D13S317: 13 D7S820: 10, 11 D16S539: 12 vWA: 15, 17 THO1: 7, 8 TPOX: 8 Amelogenin: X CSF1PO: 9,14 |
| Population Doubling Time： | approximately 72 hours |
| Name of Depositor： | S. Rosen |
| Year of Origin： | 1986 |
| References： | Goldman-Leikin RE, et al. Characterization of a novel myeloma cell line, MM.1. J. Lab. Clin. Med. 113(3):335-345, 1989. PubMed: 2926241 Greenstein S, et al. Characterization of the MM.1 human multiple myeloma (MM) cell lines: A model system to elucidate the characteristics, behavior, and signaling of steroid-sensitive and -resistant MM cells. Exp. Hematol.: 31:271-282, 2003. PubMed; 12691914 Moalli PA, et al. A mechanism of resistance to glucocorticoids in multiple myeloma: transient expression of a truncated glucocorticoid receptor mRNA. Blood. 79:213-222, 1992. PubMed: 1728309 Greenstein S, et al. Characterization of the MM.1 human multiple myeloma (MM) cell lines: A model system to elucidate the characteristics, behavior, and signaling of steroid-sensitive and -resistant MM cells. Exp. Hematol.: 31:271-282, 2003. PubMed; 12691914 |