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微信小章| 产品名称： | C6 |
|---|---|
| 商品货号： | TS209451 |
| Organism： | Rattus norvegicus, rat |
| Tissue： | brain |
| Cell Type： | glial cell |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | glioma |
| Applications： | This cell line is a suitable transfection host. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | Stemline number is diploid. Karyotype is stable within the stemline number and is that of a normal male. Three cells with breaks; one with a secondary constriction, one with a dicentric, one with a rearrangement and four with terminal or centromere associations. Note: Cytogenetic information is based on initial seed stock at ATCC. Cytogenetic instability has been reported in the literature for some cell lines. |
| Derivation： | The glial cell strain, C6, was cloned from a rat glial tumor induced by N-nitrosomethylurea by Benda et al. after a series of alternate culture and animal passages. |
| Receptor Expression： | glucocorticoid receptor |
| Genes Expressed： | S-100 protein; produce glyceryl phosphate dehydrogenase in response to glucocorticoids; somatotrophin |
| Cellular Products： | S-100 protein; produce glyceryl phosphate dehydrogenase in response to glucocorticoids; somatotrophin |
| Virus Susceptibility： | Vaccinia virus
Vesicular stomatitis, Orsay (Indiana) Vesicular stomatitis, Glasgow (Indiana) Herpes simplex virus |
| Virus Resistance： | poliovirus 3 |
| Comments： | S-100 production increases ten fold as cells grow from low density to confluency. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 2.5%; horse serum to a final concentration of 15%.
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| Subculturing： | Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: 2 to 3 times per week |
| Cryopreservation： | Freeze medium: culture medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
| Name of Depositor： | G Sato |
| Deposited As： | Rattus sp. |
| References： | Benda P, et al. Differentiated rat glial cell strain in tissue culture. Science 161: 370-371, 1968. PubMed: 4873531 Lightbody JJ, et al. Establishment of differentiated clonal strains of glial brain cells in culture. Fed. Proc. 27: 720, 1968. Chen Y, et al. Demonstration of binding of dengue virus envelope protein to target cells. J. Virol. 70: 8765-8772, 1996. PubMed: 8971005 |
| Cross References： | Nucleotide (GenBank) : X89603 R.norvegicus mRNA for metallothionein-III. Nucleotide (GenBank) : E01331 Rat glia-derived neurite-promoting factor gene. Nucleotide (GenBank) : AF069306 Rattus norvegicus transaldolase mRNA, complete cds. Nucleotide (GenBank) : AF202115 Rattus norvegicus GPI-anchored ceruloplasmin mRNA, complete cds. Nucleotide (GenBank) : X60002 Rat delta CREB mRNA for cAMP-responsive element (CRE) binding protein. |