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微信小章| 产品名称： | Aspergillus tubingensis (Schober) Mosseray |
|---|---|
| 商品货号： | TS209853 |
| Deposited As： | Aspergillus ficuum (Reichert) Hennings |
| Strain Designations： | SRRC 265 NRRL 3135 |
| Application： | degrades phytic acid produces 3-phytase produces acid phosphatase produces glucoamylase phytate hydrolysis in soybeans and cottonseed |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | freeze-dried |
| Storage Conditions： | Frozen: -80°C or colder Freeze-Dried: 2°C to 8°C Live Culture: See Propagation Section |
| Type Strain： | no |
| Preceptrol®： | no |
| Comments： | Immobilization of phytase
The name of this strain was changed based on in-house sequence analysis |
| Medium： | ATCC® Medium 28: Emmons modification of Sabourauds agar ATCC® Medium 325: Malt extract agar (Blakeslees formula) ATCC® Medium 336: Potato dextrose agar (PDA) |
| Growth Conditions： | Temperature: 24°C to 26°C Atmosphere: Typical aerobic |
| Sequenced Data： |
18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28/26S ribosomal RNA gene, partial sequence AAGGATCATTACCGAGTGCGGGTCCTTTGGGCCCAACCTCCCATCCGTGTCTATTATACCCTGTTGCTTCGGCGGGCCCGCCGCTTGTCGGCCGCCGGGGGGGCGCCTTTGCCCCCCGGGCCCGTGCCCGCCGGAGACCCCAACACGAACACTGTCTGAAAGCGTGCAGTCTGAGTTGATTGAATGCAATCAGTTAAAACTTTCAACAATGGATCTCTTGGTTCCGGCATCGATGAAGAACGCAGCGAAATGCGATAACTAATGTGAATTGCAGAATTCAGTGAATCATCGAGTCTTTGAACGCACATTGCGCCCCCTGGTATTCCGGGGGGCATGCCTGTCCGAGCGTCATTGCTGCCCTCAAGCCCGGCTTGTGTGTTGGGTCGCCGTCCCCCTCTCCGGGGGGACGGGCCCGAAAGGCAGCGGCGGCACCGCGTCCGATCCTCGAGCGTATGGGGCTTTGTCACATGCTCTGTAGGATTGGCCGGCGCCTGCCGACGTTTTCCAACCATTTTTTCCAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAAG D1D2 region of the 28/26S ribosomal RNA gene CATATCAATAAGCGGAGGAAAAGAAACCAACCGGGATTGCCTCAGTAACGGCGAGTGAAGCGGCAAGAGCTCAAATTTGAAAGCTGGCTCCTTCGGAGTCCGCATTGTAATTTGCAGAGGATGCTTTGGGTGCGGCCCCCGTCTAAGTGCCCTGGAACGGGCCGTCAGAGAGGGTGAGAATCCCGTCTTGGGCGGGGTGTCCGTGCCCGTGTAAAGCTCCTTCGACGAGTCGAGTTGTTTGGGAATGCAGCTCTAAATGGGTGGTAAATTTCATCTAAAGCTAAATACTGGCCGGAGACCGATAGCGCACAAGTAGAGTGATCGAAAGATGAAAAGCACTTTGAAAAGAGAGTTAAACAGCACGTGAAATTGTTGAAAGGGAAGCGCTTGCGACCAGACTCGCCCGCGGGGTTCAGCCGGCATTCGTGCCGGTGTACTTCCCCGTGGGCGGGCCAGCGTCGGTTTGGGCGGCCGGTCAAAGGCCCCTGGAATGTAGTACCCTCCGGGGTACCTTATAGCCAGGGGTGCAATGCGGCCAGCCTGGACCGAGGAACGCGCTTCGGCACGGACGCTGGCATAATGGTCGTAAACGA |
| Name of Depositor： | AH Ullah |
| Chain of Custody： | ATCC <-- AH Ullah <-- R.J. Wodzinski |
| Cross References： | Nucleotide (GenBank) : L20567 phytase B gene Nucleotide (GenBank) : U18553 Aspergillus ficuum TS209853 acid phosphatase (aphA) gene, |
| References： | Ullah AH. Production, rapid purification and catalytic characterization of extracellular phytase from Aspergillus ficuum. Prep. Biochem. 18: 443-458, 1988. PubMed: 2852806 Ullah AH. Aspergillus ficuum phytase: partial primary structure, substrate selectivity, and kinetic characterization. Prep. Biochem. 18: 459-471, 1988. PubMed: 2852807 Ullah AH, Phillippy BQ. Immobilization of Aspergillus ficuum phytase: product characterization of the bioreactor. Prep. Biochem. 18: 483-489, 1988. PubMed: 2852808 Ullah AH, Gibson DM. Extracellular phytase from Aspergillus ficuum NRRL 3135. Purification and characterization. Prep. Biochem. 17: 63-91, 1987. PubMed: 3035533 Ullah AH, Cummins BJ. Aspergillus ficuum extracellular phytase. Immobilization on glutaraldehyde-activated silicate. Ann. N.Y. Acad. Sci. 542: 102-106, 1988. . . Bioresour. Technol. 53: 7-12, 1995. Ehrlich KC, et al. Identification and cloning of a second phytase gene (phyB) from Aspergillus niger (ficuum). Biochem. Biophys. Res. Commun. 195: 53-57, 1993. PubMed: 7916610 Vandersall AS, et al. Identification, characterization, and partial purification of glucoamylase from Aspergillus niger (syn A. ficuum) NRRL 3135. Prep. Biochem. 25: 29-55, 1995. PubMed: 7603971 . . Appl. Microbiol. Biotechnol. 34: 183-188, 1990. Agric. Food Chem. 42: 423-425, 1994. . . Bioresour. Technol. 54: 241-247, 1995. J. Agric. Food Chem. 36: 259-262, 1988. Hamada JS. Use of polyethylene glycol and high-performance liquid chromatography for preparative separation of Aspergillus ficuum acid phosphatases. J. Chromatogr. 658: 371-380, 1994. PubMed: 8118550 |