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微信小章| 产品名称： | Tolypocladium inflatum Gams |
|---|---|
| 商品货号： | TS209921 |
| Deposited As： | Trichoderma polysporum (Link : Persoon) Rifai |
| Strain Designations： | NRRL 8044 FERM-P 2796 |
| Alternate State： | Cordyceps subsessilis Petch, teleomorph |
| Application： | Produces chitinase
Produces cyclosporin A S-7481/F-1
Produces cyclosporin B S-7481/F-2
Produces cyclosporin C
Produces cyclosporin D
Produces cyclosporin E
Produces cyclosporin G
Produces cyclosporin synthetase
Produces dihydrocyclosporin C
Produces dihydrocyclosporin G
Produces isocyclosporin G
Produces lipase
Transformation host |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | freeze-dried |
| Storage Conditions： | Frozen: -80°C or colder Freeze-Dried: 2°C to 8°C Live Culture: See Propagation Section |
| Type Strain： | no |
| Preceptrol®： | no |
| Genome Sequenced Strain： | Yes |
| Comments： | Genome sequencing strain (Oregon State University, USA) |
| Medium： | ATCC® Medium 200: YM agar or YM broth ATCC® Medium 336: Potato dextrose agar (PDA) |
| Growth Conditions： | Temperature: 24°C to 26°C Atmosphere: Typical aerobic |
| Sequenced Data： |
18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence GGTCTCCGTTGGTGAACCAGCGGAGGGATCATTACCGAGTTATCAACTCCCAAACCCCTGTGAACATACCCAACGTTGCTTCGGCGGGACCGCCCCGGCGCCTCGGCGTCCCGGAACCAGGCGCCCGCCGGAGGACCCAAACTCTTGTTTAACCATAGTGGCATATTCTGAGTCTCACAAGAAAAATGAATCAAAACTTTCAACAACGGATCTCTTGGCTCTGGCATCGATGAAGAACGCAGCGAAATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCGCCAGTATTCTGGCGGGCATGCCTGTTCGAGCGTCATTTCAACCCTCAAGCCCCAGCGGCTTGGTGTTGGGGACCGGCCCCGGCCGCCCCCCAAATGCAGTGGCGACCTCGCCGCAGCCTCCCCTGCGTAGTAGCACAACTCGCACCGGAGCGCGGAGACGGTCACGCCGTAAAACGCCCAACTTCTCAGAGTTGACCTCGGATCAGGTAGGAATACCCGCTGAACTTAAGCATATCAATAA D1D2 region of the 28S ribosomal RNA gene ATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCCCCAGTAACGGCGAGTGAAGCGGCAACAGCTCAAATTTGAAATCTGGCCCCCAGGGCCCGAGTTGTAATTTGCAGAGGATGCTTTTGGCGCGGCGCCTTCCGAGTTCCCTGGAACGGGACGCCATAGAGGGTGAGAGCCCCGTCTGGTCGGACGCCAAGCCAGTGTAAAGCTCCTTCGACGAGTCGAGTAGTTTGGGAATGCTGCTCTAAATGGGAGGTATATGTCTTCTAAAGCTAAATATAGGCCAGAGACCGATAGCGCACAAGTAGAGTGATCGAAAGATGAAAAGCACTTTGAAAAGAGGGTTAAACAGTACGTGAAATTGTTGAAAGGGAAGCACTTGTGACCAGACTTGGGCCCGGTGAATCATCCAGCGTTCTCGCTGGTGCACTTCGCCGGGCCCAGGCCAGCATCAGTTCGCCGCGGGGGATAAAAGCTTCGGGAACGTAGCTCCCTCGGGAGTGTTATAGCCCGTTGCATAATACCCTGCGGTGGACTGAGGTTCGCGCTCCGCAAGGATGCTGGCGTAATGGTCACCAGTGAC |
| Name of Depositor： | NRRL |
| Isolation： | Soil |
| Cross References： | Nucleotide (GenBank) : Z69598 CPA-element Nucleotide (GenBank) : AJ295083 5.8S rRNA and ITS2 Nucleotide (GenBank) : Z28383 cyclosporin synthetase gene Nucleotide (GenBank) : A36768 Sequence 1 from Patent EP0578616. Nucleotide (GenBank) : A36772 Sequence 5 from Patent EP0578616. Nucleotide (GenBank) : A40404 Sequence 31 from Patent WO9425606. Nucleotide (GenBank) : A40406 Sequence 33 from Patent WO9425606. Nucleotide (GenBank) : A40408 Sequence 35 from Patent WO9425606. Nucleotide (GenBank) : A40409 Sequence 36 from Patent WO9425606. Nucleotide (GenBank) : A40410 Sequence 37 from Patent WO9425606. Nucleotide (GenBank) : S80937 lamda clone 6-14, repetitive element Nucleotide (GenBank) : AJ251864 partial tnir1 gene for pathway-specific nitrogen regulator, exons 1-2 Nucleotide (GenBank) : AOHE00000000 Tolypocladium inflatum NRRL8044, whole genome shotgun sequencing project |
| References： | Offenzeller M, et al. Biosynthesis of the unusual amino acid (4R)-4-(E)-2-butenyl-4-methyl-L-threonine of cyclosporin A. J. Biol. Chem. 268: 26127-26134, 1993. PubMed: 8253730 Ruegger A, Kuhn M. Dihydrocyclosporin C. US Patent 4,108,985 dated Aug 22 1978 Harri E, Ruegger A. Organic compounds. US Patent 4,117,118 dated Sep 26 1978 Traber RP, et al. Cyclosporin derivatives, their production and pharmaceutical compositions containing them. US Patent 4,288,431 dated Sep 8 1981 Weber G, et al. The peptide synthetase catalyzing cyclosporine production in Tolypocladium niveum is encoded by a giant 45.8-kilobase open reading frame. Curr. Genet. 26: 120-125, 1994. PubMed: 8001164 Weber G, Leitner E. Disruption of the cyclosporin synthetase gene of Tolypocladium niveum. Curr. Genet. 26: 461-467, 1994. PubMed: 7874740 von Arx JA. Tolypocladium, a synomym of Beauveria. Mycotaxon 25: 153-158, 1986. Kempken F, et al. A unique repeated DNA sequence in the cyclosporin-producing strain of Tolypocladium inflatum (TS209921). Exp. Mycol. 19: 305-313, 1995. PubMed: 8574905 Agathos SN, Lee J. Mathematical modeling of the production of cyclosporin A by Tolypocladium inflatum: effect of L-valine. Biotechnol. Prog. 9: 54-63, 1993. PubMed: 7763411 Kempken F, Kuck U. Evidence for circular transposition derivatives from the fungal hAT-transposon Restless. Curr. Genet. 34: 200-203, 1998. PubMed: 9745022 Lee TH, et al. Development of a cell-loaded biosupport separator for continuous immobilized-cell perfusion culture. Biotechnol. Prog. 15: 267-272, 1999. PubMed: 10194403 Ruegger A, et al. Cyclosporin A, a Peptide Metabolite from Trichoderma polysporum (Link ex Pers.) Rifai, with a remarkable immunosuppressive activity. Helv. Chim. Acta 59: 1075-1092, 1976. PubMed: 950308 Traber R, et al. New cyclopeptides from Trichoderma polysporum (Link ex Pers.) Rifai: cyclosporins B, D and E. Helv. Chim. Acta 60: 1568-1578, 1977. PubMed: 561044 Hoppert M, et al. Structure and localization of cyclosporin synthetase, the key enzyme of cyclosporin biosynthesis in Tolypocladiium inflatum. Arch. Microbiol. 176: 285-293, 2001. PubMed: 11685373 Lee J, Agathos SN. Effect of amino acids on the production of cyclosporin A by Tolypocladium inflatum. Biotechnol. Lett. 11: 77-82, 1989. Aarnio TH, Agathos SN. Production of extracellular enzymes and cyclosporin by Tolypocladium inflatum and morphologically related fungi. Biotechnol. Lett. 11: 759-764, 1989. Windhofer F, et al. Ds-like restless deletion derivatives occur in Tolypocladium inflatum and two foreign hosts, Neurospora crassa and Penicillium chrysogenum. Fungal Genet Biol 35: 171-182, 2002. PubMed: 11848679 |