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微信小章| 产品名称： | Penicillium rubens Biourge |
|---|---|
| 商品货号： | TS210129 |
| Deposited As： | Penicillium notatum Westling |
| Strain Designations： | NRRL 832 ATCC 7813, B 69, CBS 197.46, IMI 17968, QM 940 |
| Application： | Produces penicillin
Produces phosphogluconate dehydrogenase 6-phosphogluconate dehydrogenase |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | freeze-dried |
| Storage Conditions： | Frozen: -80°C or colder Freeze-Dried: 2°C to 8°C Live Culture: See Propagation Section |
| Type Strain： | no |
| Preceptrol®： | yes |
| Comments： | Prototype strain for production of penicillin in submerged culture The strain was reidentified as Penicillium rubens based on the multiloci DNA sequence analysis |
| Medium： | ATCC® Medium 28: Emmons modification of Sabourauds agar ATCC® Medium 312: Czapeks agar ATCC® Medium 336: Potato dextrose agar (PDA) |
| Growth Conditions： | Temperature: 25°C to 30°C Atmosphere: Typcial aerobic |
| Sequenced Data： |
18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence
GGTTTCCGTAGGTGAACCTGCGGAAGGATCATTACCGAGTGAGGGCCCTCTGGGTCCAACCTCCCACCCGTGTTTATTTTACCTTGTTGCTTCGGCGGGCCCGCCTTAACTGGCCGCCGGGGGGCTTACGCCCCCGGGCCCGCGCCCGCCGAAGACACCCTCGAACTCTGTCTGAAGATTGTAGTCTGAGTGAAAATATAAATTATTTAAAACTTTCAACAACGGATCTCTTGGTTCCGGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCAAATTCAGTGAATCATCGAGTCTTTGAACGCACATTGCGCCCCCTGGTATTCCGGGGGGCATGCCTGTCCGAGCGTCATTTCTGCCCTCAAGCACGGCTTGTGTGTTGGGCCCCGTCCTCCGATCCCGGGGGACGGGCCCGAAAGGCAGCGGCGGCACCGCGTCCGGTCCTCGAGCGTATGGGGCTTTGTCACCCGCTCTGTAGGCCCGGCCGGCGCTTGCCGATCAACCCAAATTTTTATCCAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAAGCATATCAATAA Beta-tubulin (TUB2) gene, partial sequence GATCCCCTCCAGACGCGTCTTTTGTTTTCATATGGCCCCTGAGCCACGACCCCACCCCCAACAGATCTTTTGCTAACTTGCTCTAGGTTCACCTCCAAACCGGCCAGTGTGTAAGTTCAACATGGAACATTCTTGGAAACATTCTTCGATTCGTGGGACTAAACTGGAATTGGGTTATAGGGTAACCAAATTGGTGCCGCTTTCTGGTAAGTCTCGGAGCTTTTTTTTTCGCGTTGGGTATCAATTGACAAGTTGCTAACTGGATTACAGGCAAACCATCTCTGGCGAGCACGGTCTCGATGGCGATGGACAGTAAGTTTAACAGTGATAGGGATTCTGGTGGATCACACGTCTGATATCTTGCTAGGTACAATGGTACCTCCGACCTCCAGCTCGAGCGTATGAACGTCTACTTCAACCATGTGAGTACAATGACTGGGAATCGATTGATTGTGCATCATCTGATCGGACGTTTTTCTTTGACAATCTAGGCCAGCGGTGACAAGTACGTTCCCCGTGCCGTTCTGGTCGATTTGGAGCCCGGTACCATGGATGCTGTCCGCTCCGGTCCCTTCGGCAAGCTTTTCCGCCCCGACAACTTCGTCTTCGGTCAGTCCGGTGCTGGTAACAACTGGGCCAAGGGTCACT D1D2 region of the 28S ribosomal RNA gene ATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCCCCAGTAACGGCGAGTGAAGCGGCAAGAGCTCAAATTTGAAAGCTGGCTCCTTCGGGGTCCGCATTGTAATTTGTAGAGGATGCTTCGGGAGCGGTCCCCATCTAAGTGCCCTGGAACGGGACGTCATAGAGGGTGAGAATCCCGTATGGGATGGGGTGTCCGCGCCCGTGTGAAGCTCCTTCGACGAGTCGAGTTGTTTGGGAATGCAGCTCTAAATGGGTGGTAAATTTCATCTAAAGCTAAATATTGGCCGGAGACCGATAGCGCACAAGTAGAGTGATCGAAAGATGAAAAGCACTTTGAAAAGAGAGTTAAAAAGCACGTGAAATTGTTGAAAGGGAAGCGCTTGCGACCAGACTCGCTCGCGGGGTTCAGCCGGCATTCGTGCCGGTGTACTTCCCCGCGGGCGGGCCAGCGTCGGTTTGGGCGGTCGGTCAAAGGCCCTCGGAAGGTAACGCCCCTAGGGGCGTCTTATAGCCGAGGGTGCAATGCGACCTGCCTAGACCGAGGAACGCGCTTCGGCTCGGACGCTGGCATAATGGTCGTAAACGAC |
| Name of Depositor： | NRRL |
| Chain of Custody： | ATCC |
| Isolation： | Contaminant in culture labeled P. lacticus Maze & Perrier from Ph. Biourge 69, Washington, DC |
| Cross References： | Nucleotide (GenBank) : KU729086 ITS including 5.8S rRNA gene Nucleotide (GenBank) : KU729184 D1/D2 region of 28S rRNA gene Nucleotide (GenBank) : KU896998 beta-tubulin (TUB2) gene |
| References： | Houbraken J, et al. New penicillin-producing Penicillium species and an overview of section Chrysogena. Persoonia 29: 78-100, 2012. PubMed: 23606767 Miller HM, Shepherd MG. Purification and properties of 6-phosphogluconate dehydrogenase from Penicillium duponti and Penicillium notatum. Can. J. Microbiol. 18: 1289-1298, 1972. PubMed: 5052896 Moyer AJ, Coghill RD. Penicillin. IX. The laboratory scale production of penicillin in submerged cultures by Penicillium notatum Westling (NRRL 832). J. Bacteriol. 51: 79-93, 1946. Chou H, et al. Transaldolases are novel and immunoglobulin E cross-reacting fungal allergens. Clin. Exp. Allergy 41: 739-749, 2011. PubMed: 21488999 Houbraken J, Frisvad JC, Samson RA. Flemings penicillin producing strain is not Penicillium chrysogenum but P. rubens. IMA Fungus 2: 87-95, 2011. PubMed: 22679592 Chow LP, et al. Characterization of Pen n 13, a major allergen from the mold Penicillium notatum. Biochem. Biophys. Res. Commun. 269: 14-20, 2000. PubMed: 10694469 |