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微信小章| 产品名称： | Galactomyces candidus de Hoog et Smith |
|---|---|
| 商品货号： | TS210298 |
| Deposited As： | Geotrichum candidum Link : Persoon |
| Application： | Degrades sauerkraut brine Produces acetoin dehydrogenase diacetyl reductase Produces lactate dehydrogenase Produces lipase Produces polygalacturonase endopolygalacturonase, pectinase Quality control strain Produces lipases I and II Produces type A lipase Produces extracellular lipase from sauerkraut brine Produces acetoin dehydrogenase from sauerkraut brine |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | freeze-dried |
| Storage Conditions： | Frozen: -80°C or colder Freeze-Dried: 2°C to 8°C Live Culture: See Propagation Section |
| Type Strain： | no |
| Preceptrol®： | yes |
| Comments： | Crystal structure of lipase cDNA clone of lipase genes The strain was recently re-identified as Galactomyces candidus (anamorphic synonym: Geotrichum candidum) based on the multigene sequence analysis. |
| Morphology： | On YM medium at 25°C after 3 days, colonies white to dingy white, finely hairy (able to see the branching hyphae in the texture), dense, raised. Hyphae hyaline, guttulate. Arthroconidia rectangular to cubic, hyaline, smooth, 6-7.5 X 3-3.75 µm. Yeast cells subglobose to oval, hyaline, smooth, larger cells 9-12 X 4.5-8.25 µm, smaller cells 5.25-6 X 4.5-5.25 µm. |
| Medium： | ATCC® Medium 325: Malt extract agar (Blakeslees formula) ATCC® Medium 200: YM agar or YM broth ATCC® Medium 1245: YEPD |
| Growth Conditions： | Temperature: 24°C to 26°C Atmosphere: Typical aerobic |
| Sequenced Data： |
18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence
TCAAAACAAAAATAATCAAAACTTTTAACAATGGATCTCTTGGTTCTCGTATCGATGAAGAACGCAGCGAAACGCGATATTTCTTGTGAATTGCAGAAGTGAATCATCAGTTTTTGAACGCACATTGCACTTTGGGGTATCCCCCAAAGTATACTTGTTTGAGCGTTGTTTCTCTCTTGGAATTGCATTGCTTTTCTAAAATTTCGAATCAAATTCGTTTGAAAAACAACACTATTCAACCTCAGATCAAGTAGGATTACCCGCTGAACTTAA D1D2 region of the 28S ribosomal RNA gene ATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCCTTAGTAACGGCGAGTGAAGCGGCAAAAGCTCAAATTTGAAATCGGCCACCAGGTCGAGTTGTAATTTGTAGATTGTATCTTGAGAGCGGATTAAAGTCTGTTGGAACACAGCGCCTTAGAGGGTGACAGCCCCGTAAAATCTATTCTCATTGTAAGATACTTTCGAAGAGTCGAGTTGTTTGGGAATGCAGCTCTAAGTGGGAGGTAAATTCCTTCTAAAGCTAAATATTGACGAGAGACCGATAGCGAACAAGTACTGTGAAGGAAAGATGAAAAGCACTTTGAAAAGAGAGTGAAAAAGTACGTGAAATTGTTAAAAGGGAAGGGTATTGAATCAGACTTGGTGCTGTTGTTCAACTGTGTTTCGGCAYAGTGTACTCAGCAGTACTAGGCCAAGGTGGGGTGTTTGGGAGTGAAAAAGAAGTWGGAACGTAACTCTTCGGAGTGTTATAGCCTACTTTCATAGCTCCTCAGGCGCCTCAGGACTGCGCTTCGGCAAGGACCTTGGCATAATGATTCTATACCG Actin gene ATCACACTTTTTACAATGAGCTCAGAGTTGCTCCCGAGGAGCACCCTGTCCTCCTGACTGAGGCCCCCGTCAACCCCAAGTCCAACAGAGAGAAGATGACCCAGATTGTCTTTGAGACCTTCAACGCCCCTGCTTTCTACGTTTCTATCCAGGCCGTTCTTTCCCTTTACGCCTCTGGTCGTACCACTGGTATCGTTCTCGATTCCGGTGATGGTGTCACCCACGTTGTCCCCATTTACGCCGGTTTCTCCCTCCCCCACGCCATTATGCGTATTGACCTGGCTGGCCGTGACTTGACCGACTACCTCATGAAGATTCTTACTGAGAGAGGTTACTCTTTCTCCACCTCTGCCGAGCGTGAAATTGTCCGTGAGATTAAGGAGAAGCTCTGTTACGTTGCTCTTGACTTTGAGCAGGAGCTCCAGACTGCTGCCGAGTCCTCCACTCTGGAAAAGAGCTACGAGCTTCCTGATGGTCAGGTCATCACCATTGGTAACGAGAGATTCAGAGCTCCTGAGGCTCTCTTCCACCCCTCTGTCCTGGGTCTTGAGGCTGCTGGTATTGATGAGACCACTTACAACTCTATCATGAAGTGTGATGTTGATGTCCGTAAGGATCTTTACGGTAACATTGTCATGTCTGGTGGTACCACCATGTACCCCGGTATTGCCGAGCGTATGCAGAAGGAGATTACTGCTCTTGCTCCTTCTTCCATGAAGGTCAAGATCATTGCTCCTCCCGAGAGAAAGTTCTCTGTCTGGATTGGTGGTTCCATTCTTGCTTCCCTCTCCACCTTCCAACAGATGTGGATCTCCAAGCAGGAGTA |
| Name of Depositor： | M Iwai |
| Isolation： | Clotted carrot, Japan |
| Cross References： | Nucleotide (GenBank) : KU933438 ITS including 5.8S rRNA gene Nucleotide (GenBank) : KU933412 D1/D2 region of 28S rRNA gene Nucleotide (GenBank) : KU933403 actin gene Nucleotide (GenBank) : E02497 cDNA encoding Geotrichum candidum lipase. Nucleotide (GenBank) : U02622 Geotrichum candidum TS210298 lipase gene, partial cds. Nucleotide (GenBank) : U26858 Galactomyces geotrichum isolate g56 28S rRNA gene, partial Nucleotide (GenBank) : U02541 Geotrichum candidum TS210298 lipase II gene, partial cds. |
| References： | . . Food Chem. 45: 15-17, 1992. Hang YD, Woodams EE. Production and characterization of polygalacturonase from Geotrichum candidum. World J. Microbiol. Biotechnol. 8: 480-482, 1992. Jacobsen T, Poulsen OM. Comparison of lipases from different strains of the fungus Geotrichum candidum. Biochim. Biophys. Acta 1257: 96-102, 1995. PubMed: 7619863 Shimada Y, et al. cDNA molecular cloning of Geotrichum candidum lipase. J. Biochem. 106: 383-388, 1989. PubMed: 2481674 Nagao T, et al. Cloning and sequencing of two chromosomal lipase genes from Geotrichum candidum. J. Biochem. 113: 776-780, 1993. PubMed: 8370674 Schrag JD, et al. Multiple crystal forms of lipases from Geotrichum candidum. J. Mol. Biol. 220: 541-543, 1991. PubMed: 1870119 . . Bioresour. Technol. 43: 181-183, 1993. Hata Y, et al. Low resolution crystal structure of lipase from Geotrichum candidum (ATCC34614). J. Biochem. 86: 1821-1827, 1979. PubMed: 575138 Sugihara A, et al. Separation and characterization of two molecular forms of Geotrichum candidum lipase. J. Biochem. 107: 426-430, 1990. PubMed: 2341377 Shimada Y, et al. cDNA cloning and characterization of Geotrichum candidum lipase II. J. Biochem. 107: 703-707, 1990. PubMed: 2398037 Catoni E, et al. Overexpression of lipase A and B of Geotrichum candidum in Pichia pastoris: high-level production and some properties of functional expressed lipase B. Biotechnol. Tech. 11: 689-695, 1997. Iwai M, et al. Lipid requirement for the lipase production by Geotrichum candidum Link. Agric. Biol. Chem. 37: 929-931, 1973. Iwai M, et al. Synthesis of terpene alcohol esters by lipase. Agric. Biol. Chem. 44: 2731-2732, 1980. Hang YD, Woodams EE. Lipase production by Geotrichum candidum from sauerkraut brine. World J. Microbiol. Biotechnol. 6: 418-421, 1990. FF MicroPlate. Biolog. YT MicroPlate QC Set. Biolog. Arteau M, Labrie S, Roy D. Terminal-restriction fragment length polymorphism and automated ribosomal intergenic spacer analysis profiling of fungal communities in Camembert cheese. Int. Dairy J. 20: 545-554, 2010. |