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微信小章| 产品名称： | ZR-75-1 |
|---|---|
| 商品货号： | TS210618 |
| Organism： | Homo sapiens, human |
| Tissue： | mammary gland; breast/duct; derived from metastatic site: ascites |
| Cell Type： | epithelial |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | ductal carcinoma |
| Age： | 63 years adult |
| Gender： | female |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | This human cell line has a hypertriploid chromosome number. The modal chromosome number was 72 occurring in 26% of the cells and the rate of higher ploidies was at 1.2%. Eighteen markers were common.They are: t(1q,?), M2,M3, del(3) (p21), der(5)t(5;?) (q35;?), del(6) (q21), t(11q14q), t(11;11) (p15;q11), der(14)t(2;14) (q21;q32), t(17q,?), M13, M14, M15, M16, M17, der(8)t(8;?), t(9p,?) and 19pt. Of these M13 was paired. Normal N14 was not found. N6 was single copied, X had 3 copies and N18 had 4 copies in each cell. |
| Images： |  |
| Clinical Data： | 63 years adult Caucasian female |
| Receptor Expression： | estrogen receptor, expressed |
| Genes Expressed： | mucin (apomucin, MUC-1, MUC-2) |
| Cellular Products： | mucin (apomucin, MUC-1, MUC-2) |
| Tumorigenic： | Yes |
| Effects： | Yes, forms tumors in nude mice |
| Comments： | The cells produce high levels of MUC-1 mucin mRNA, low levels of MUC-2 mRNA but do not express the MUC-3 gene. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
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| Subculturing： | Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.xa0Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| STR Profile： | Amelogenin: X CSF1PO: 10,11 D13S317: 9 D16S539: 11 D5S818: 13 D7S820: 10,11 THO1: 7,9.3 TPOX: 8 vWA: 16,18 |
| Isoenzymes： | G6PD, B |
| Population Doubling Time： | 80 hrs |
| Name of Depositor： | LW Engel |
| Deposited As： | Homo sapiens |
| References： | Engel LW, Young NA. Human breast carcinoma cells in continuous culture: a review. Cancer Res. 38: 4327-4339, 1978. PubMed: 212193 Engel LW, et al. Establishment and characterization of three new continuous cell lines derived from human breast carcinomas. Cancer Res. 38: 3352-3364, 1978. PubMed: 688225 Dahiya R, et al. Mucin synthesis and secretion in various human epithelial cancer cell lines that express the MUC-1 mucin gene. Cancer Res. 53: 1437-1443, 1993. PubMed: 8443822 Bellet D, et al. Malignant transformation of nontrophoblastic cells is associated with the expression of chorionic gonadotropin beta genes normally transcribed in trophoblastic cells. Cancer Res. 57: 516-523, 1997. PubMed: 9012484 Couillard S, et al. Comparison of the effects of the antiestrogens EM-800 and tamoxifen on the growth of human breast ZR-75-1 cancer xenografts in nude mice. Cancer Res. 58: 60-64, 1998. PubMed: 9426058 Wang E, et al. Posttranscriptional regulation of protein expression in human epithelial carcinoma cells by adenine-uridine-rich elements in the 3-untranslated region of tumor necrosis factor-alpha messenger RNA. Cancer Res. 57: 5426-5433, 1997. PubMed: 9393771 Littlewood-Evans AJ, et al. The osteoclast-associated protease cathepsin K is expressed in human breast carcinoma. Cancer Res. 57: 5386-5390, 1997. PubMed: 9393764 Landers JE, et al. Translational enhancement of mdm2 oncogene expression in human tumor cells containing a stabilized wild-type p53 protein. Cancer Res. 57: 3562-3568, 1997. PubMed: 9270029 Sheng S, et al. Maspin acts at the cell membrane to inhibit invasion and motility of mammary and prostatic cancer cells. Proc. Natl. Acad. Sci. USA 93: 11669-11674, 1996. PubMed: 8876194 |
| Cross References： | Nucleotide (GenBank) : L38933 Homo sapiens GT198 mRNA, complete ORF. Nucleotide (GenBank) : U19769 Human CENP-F kinetochore protein mRNA, complete cds. Nucleotide (GenBank) : NM_013290 Homo sapiens GT198, complete ORF (HUMGT198A), mRNA. Nucleotide (GenBank) : NM_005196 Homo sapiens centromere protein F (350/400kD, mitosin) (CENPF),mRNA Nucleotide (GenBank) : U48296 Homo sapiens protein tyrosine phosphatase PTPCAAX1 (hPTPCAAX1) mRNA, complete cds. Nucleotide (GenBank) : U48297 Homo sapiens protein tyrosine phosphatase PTPCAAX2 (hPTPCAAX2) mRNA, complete cds. |