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微信小章| 产品名称： | YPEN-1 |
|---|---|
| 商品货号： | TS210620 |
| Organism： | Rattus norvegicus, rat |
| Tissue： | prostate, endothelium |
| Cell Type： | immortalized with adenovirus 12 - SV40 virus hybrid (Ad12-SV40) |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 2 Cells contain polyomavirus DNA sequences
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | normal |
| Age： | 8 weeks |
| Gender： | male |
| Strain： | Copenhagen |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | The YPEN-1 cell line was originated in 1993 from prostate cells of 8 week old Copenhagen male rats. |
| Tumorigenic： | No |
| Effects： | No, the cells were not tumorigenic in immunosuppressed mice, but did form colonies in semisolid medium. |
| Comments： | Cells were cultured in Endothelium Isolation Medium and immortalized by infection with Adenovirus12 SV40 hybrid virus. The cells stain positively for but are non-producers of SV40 T-antigen. YPEN-1 cells demonstrate acetylated low density lipoprotein (Dil-Ac-LDL) uptake as an endothelial marker. They exhibit positive staining for endothelin and for a monoclonal antibody to rat endothelium (MRC OX-43). They express Integrin a6 1 and Integrin 3 on their plasma membrane, and demonstrate tube formation in Matrigel. |
| Complete Growth Medium： | Minimum essential medium (Eagle) with 2 mM L-glutamine and Earles BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, supplemented with 0.03 mg/ml heparin, 95%; fetal bovine serum, 5%
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994. |
| Cryopreservation： | Freeze medium: Complete growth medium, 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C |
| Population Doubling Time： | 26 hrs |
| Name of Depositor： | K Yamakazi, K Pienta |
| Deposited As： | Rattus sp. |
| Year of Origin： | 1993 |
| References： | Yamazaki K, et al. Establishment of immortalized Copenhagen rat prostate endothelial cell lines. In Vivo 9: 421-426, 1995. PubMed: 8900918 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online. |