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购买须知
| 产品名称： | ZF4 |
|---|---|
| 商品货号： | TS210622 |
| Organism： | Danio rerio, zebrafish |
| Tissue： | embryo |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | 1 day old embryo |
| Applications： | The cells can be used as a transfection host. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | hyperploid; range = 110 to 120 |
| Images： |  |
| Derivation： | The ZF4 cell line was established from 1-day-old zebrafish embryos. |
| Comments： | The ZF4 cell line was established from 1-day-old zebrafish embryos. The cells are cultivated at 28°C.
ZF4 cells are killed within 10 days by culturing in medium containing 0.8 to 1.0 mg/mL G418. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No. 30-2006. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
NOTE:xa0DO NOT USExa0EDTA TO DISSOCIATE THExa0CELLS. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommendedMedium Renewal: 2 to 3 times per week |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 28°C |
| Name of Depositor： | W Driever |
| Deposited As： | Brachydanio rerio |
| References： | Driever W, Rangini Z. Characterization of a cell line derived from zebrafish (Brachydanio rerio) embryos. In Vitro Cell. Dev. Biol. Anim. 29A: 749-754, 1993. PubMed: 8407719 Biosafety in Microbiological and Biomedical Laboratories 4th ed.; U.S. Department of Health and Human Services ;1999. |